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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effects of Aspergillus spp. exogenous fibrolytic enzymes on in vitro fermentation of tropical forages

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Author(s):
Facchini, Fernanda D. A. [1] ; Reis, Victor R. A. [2] ; Roth, Anna Paula [3] ; Magalhaes, Karla A. [3] ; Peixoto-Nogueira, Simone C. [1] ; Casagrande, Daniel R. [4] ; Reis, Ricardo A. [3] ; Polizeli, Maria de Lourdes T. M. [5]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Dept Biochem, BR-14040901 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Dept Pharmaceut Sci, BR-14040901 Ribeirao Preto, SP - Brazil
[3] State Univ Sao Paulo UNESP, Dept Anim Sci, Sao Paulo - Brazil
[4] Fed Univ Amazon UFAM, Inst Social Sci Educ & Zootecny, Parintins, AM - Brazil
[5] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, BR-14040901 Ribeirao Preto, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Journal of the Science of Food and Agriculture; v. 92, n. 12, p. 2569-2573, SEP 2012.
Web of Science Citations: 4
Abstract

BACKGROUND: Cellulose and hemicellulose are quantitatively the most important structural carbohydrates present in ruminant diets. Rumen micro-organisms produce enzymes that catalyse their hydrolysis, but the complex network formed by structural carbohydrates and lignin reduces their digestibility and restricts efficient utilisation of feeds by ruminants. This study aimed to produce two enzymatic extracts, apply them in ruminant diets to determine the best levels for ruminal digestibility and evaluate their effects on in vitro digestibility. RESULTS: In experiment 1 a two-stage in vitro technique was used to examine the effects of different enzymatic levels of Aspergillus japonicus and Aspergillus terricola on tropical forages. Enzyme addition had minor effects on corn silage at the highest enzymatic level. In experiment 2 an in vitro gas production (GP) technique was applied to determine apparent in vitro organic matter digestibility and metabolisable energy. The addition of enzymes in GP showed interesting results. Good data were obtained using sugar cane and Tifton-85 hay supplemented with extracts of A. japonicus and A. terricola respectively. CONCLUSION: Overall, the study suggests that addition of crude extracts containing exogenous fibrolytic enzymes to ruminant diets enhances the effective utilisation of ruminant feedstuffs such as forages. Copyright (c) 2012 Society of Chemical Industry (AU)