| Full text | |
| Author(s): |
Alvarenga, Elenice M.
[1]
;
Mondin, Mateus
[2]
;
Rodrigues, Vera L. C. C.
[3]
;
Andrade, Larissa M.
[2]
;
Vidal, Benedicto de Campos
[1]
;
Mello, Maria Luiza S.
[1]
Total Authors: 6
|
| Affiliation: | [1] Univ Campinas UNICAMP, Inst Biol, BR-13083862 Campinas, SP - Brazil
[2] Univ Sao Paulo, Dept Genet, Luiz de Queiroz Coll Agr ESALQ, BR-13418900 Piracicaba, SP - Brazil
[3] SUCEN, BR-13840000 Mogi Guacu, SP - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | ACTA HISTOCHEMICA; v. 114, n. 7, p. 665-672, 2012. |
| Web of Science Citations: | 2 |
| Abstract | |
The Malpighian tubule cell nuclei of male Panstrongylus megistus, a vector of Chagas disease, contain one chromocenter, which is composed solely of the Y chromosome. Considering that different chromosomes contribute to the composition of chromocenters in different triatomini species, the aim of this study was to determine the contribution of AT-, GC-, and methylated cytidine-rich DNA in the chromocenter as well as in euchromatin of Malpighian tubule cell nuclei of P. megistus in comparison with published data for Triatoma infestans. Staining with 4',6-diamidino-2-phenylindole/actinomycin D and chromomycin A(3)/distamycin, immunodetection of 5-methylcytidine and AgNOR test were used. The results revealed AT-rich/GC-poor DNA in the male chromocenter, but equally distributed AT and GC DNA sequences in male and female euchromatin, like in T. infestans. Accumulation of argyrophilic proteins encircling the chromocenter did not always correlate with that of GC-rich DNA. Methylated DNA identified by immunodetection was found sparsely distributed in the euchromatin of both sexes and at some points around the chromocenter edge, but it could not be considered responsible for chromatin condensation in the chromocenter, like in T. infestans. However, unlike in T. infestans, no correlation between the chromocenter AT-rich DNA and nucleolus organizing region (NOR) DNA was found in P. megistus. (c) 2011 Elsevier GmbH. All rights reserved. (AU) | |
| FAPESP's process: | 10/50015-6 - Chromatin structure and organization with aging and diabetes compared to induced changes in epigenetic markers |
| Grantee: | Maria Luiza Silveira Mello |
| Support Opportunities: | Research Projects - Thematic Grants |