A glass bead protocol for recovery of host cell free Ehrlichia canis and quantification by Sybr-green real-time PCR

Cardozo, G. P.; Santos, E. V.; Fachin, A. L.; Franca, S. C.; Marins, M.

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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A glass bead protocol for recovery of host cell free Ehrlichia canis and quantification by Sybr-green real-time PCR

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Author(s):	Cardozo, G. P. ^[1] ; Santos, E. V. ^[1] ; Fachin, A. L. ^[1] ; Franca, S. C. ^[1] ; Marins, M. ^{[1, 2]} Total Authors: 5
Affiliation:	^[1] Univ Ribeirao Preto, Unidade Biotecnol, Ribeirao Preto - Brazil ^[2] Heranza Biotecnol, Ribeirao Preto - Brazil Total Affiliations: 2
Document type:	Journal article
Source:	BIOCELL; v. 35, n. 1, p. 35-36, APR 2011.
Web of Science Citations:	2
Abstract
E. canis infection of the canine cell line DH82 is a routine in studies with this bacteria. A protocol for isolation of host cell free bacteria was developed based on the use of glass beads. Improvement of infection with E. canis isolated by this method was detected by real-time PCR. (AU)

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