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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Role of mast cell degranulation in the neural correlates of the immediate allergic reaction in a murine model of asthma

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Author(s):
Costa-Pinto, Frederico Azevedo ; Basso, Alexandre Salgado ; Russo, Momtchilo [3]
Total Authors: 3
Document type: Journal article
Source: BRAIN BEHAVIOR AND IMMUNITY; v. 21, n. 6, p. 783-790, Aug. 2007.
Field of knowledge: Biological Sciences - Immunology
Abstract

Experimental airway allergy in mice leads to increased activity in specific hypothalamic and amygdaloid nuclei, and behavioral changes. The experiments described here were designed to determine the role of anaphylactic antibodies, mast cell degranulation, and lung inflammation in the neural and behavioral correlates of an experimental murine asthma-like response. Animals were sensitized intraperitoneally with ovalbumin adsorbed to alum, and challenged by intranasal ovalbumin instillation or aerosol. To induce immunological tolerance, animals were fed ovalbumin in the drinking water for 5 consecutive days, along with primary sensitization. Depletion of IgE was also accomplished with a non-anaphylactic anti-IgE antibody. Mast cell degranulation was inhibited by cromolyn. In addition to BALB/c animals, C3H/HeJ mice were used for their relative resistance to lung allergic inflammation. We confirmed that ovalbumin challenge in allergic mice leads to increased activity in the paraventricular nucleus of the hypothalamus and central nucleus of the amygdala, and avoidance behavior towards an allergen-associated compartment. Moreover, these responses were precluded by oral tolerance or anti-IgE treatment, even in the presence of IgG1. Cromolyn abrogates both responses in the presence of anaphylactic antibodies. Finally, although sensitized C3H/HeJ mice did not develop airway inflammation, they exhibited brain and behavioral changes similar to BALB/c animals. The repercussions of murine allergic asthma on brain and behavior are IgE-dependent, mediated by mast cell degranulation, and do not require a pulmonary inflammatory infiltrate, suggesting that the early phase of this immediate allergic response suffices for the brain activation associated with avoidance behavior towards exposure to the allergen. (AU)

FAPESP's process: 04/14297-6 - Activation/deactivation of macrophages and CD4+ T lymphocytes in experimental asthma
Grantee:Momtchilo Russo
Support type: Research Projects - Thematic Grants
FAPESP's process: 04/14128-0 - Neuroimmunomodulation: effects of drugs, stress and cytocines on central nervous and immune systems bidirectional relationships
Grantee:João Palermo Neto
Support type: Research Projects - Thematic Grants