Characterization of microsatellite loci of Tetragonisca angustula (Hymenoptera, Apidae, Meliponini)

Author(s)

Brito, R. M. ^[1] ; Francisco, F. O. ^[1] ; Domingues-Yamada, A. M. T. ^[1] ; Goncalves, P. H. P. ^[1] ; Pioker, F. C. ^[2] ; Soares, A. E. E. ^[3] ; Arias, M. C. ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Biociencias, Dept Genet & Biol Evolut, BR-05508090 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Biociencias, Dept Ecol, BR-05508900 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, BR-14049900 Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: CONSERVATION GENETICS RESOURCES; v. 1, n. 1, p. 183-187, DEC 2009.
• Web of Science Citations: 18

Abstract

An enriched genomic library was constructed from Tetragonisca angustula, a stingless bee species widely distributed in Brazil. The library was screened using two simple-repeat oligonucleotide probes and 21 microsatellite primer pairs were designed flanking a selection of repeat sequences within positive clones. The polymorphism of the microsatellite loci was analyzed by screening a sample of 19 unrelated T. angustula workers. Fifteen out of 21 loci were shown to be polymorphic, with observed heterozygosity estimates ranging from 0.00 to 0.89. The primers were also successfully used to amplify microsatellite loci from other stingless bee species, Tetragonisca fiebrigi, Tetragonisca weyrauchi, Lestrimelitta maracaia and Schwarziana quadripunctata. The results from variability analyses suggest that the microsatellite loci isolated from T. angustula will be useful in further population studies for the species and also for other Meliponini. (AU)

• FAPESP's process: 04/15801-0 - Biodiversity and sustainable use of pollinators, with emphasis on Meliponini bees
• Grantee: Vera Lucia Imperatriz-Fonseca
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants