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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Assessment of intraradicular bacterial composition by terminal restriction fragment length polymorphism analysis

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Author(s):
Saito, D. [1] ; Marsh, T. L. [2] ; Cannavan, F. de Souza [3] ; Hoefling, J. F. [1] ; Goncalves, R. B. [1]
Total Authors: 5
Affiliation:
[1] Univ Estadual Campinas, Dept Oral Diag, Div Microbiol & Immunol, Piracicaba Dent Sch, BR-13414903 Sao Paulo - Brazil
[2] Michigan State Univ, Ctr Microbial Ecol, Dept Microbiol & Mol Genet, E Lansing, MI 48824 - USA
[3] Univ Sao Paulo, Lab Cell & Mol Biol, Ctr Nucl Energy Agr, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Oral Microbiology and Immunology; v. 24, n. 5, p. 369-376, OCT 2009.
Web of Science Citations: 6
Abstract

Background: The aim of the study was to assess the bacterial community structures associated with endodontic infections using terminal restriction fragment length polymorphism (T-RFLP), and to investigate the correlation of whole community profiles with the manifestation of particular clinical features. Methods: Intraradicular samples were collected from 34 subjects and classified into three study groups based on the observed clinical symptoms: acute (n = 16), sub-acute (n = 8), and asymptomatic (n = 10). Genomic DNA was extracted from each sample, submitted to polymerase chain reaction using a fluorescently labeled 16S ribosomal DNA forward primer, and digested with two tetrameric endonucleases (HhaI and MspI). The terminal restriction fragments (T-RFs) were subsequently discriminated in an automated DNA sequencer, and the results were filtered using a statistics-based criterion. Results: Totals of 138 (HhaI) and 145 (MspI) unique T-RFs were detected (means 13.1 and 11.9) and there was high inter-subject variability in the bacterial assemblages. Odds-ratio analysis unveiled the existence of higher order groups of positively associated T-RFs, restating the concept that intricate ecological relationships may take place in the root canal space. A significantly greater T-RF prevalence was detected in acute cases, suggesting a straight correlation between species richness and spontaneous pain. Conclusion: Overall, no T-RFLP profile representing a specific bacterial consortium could be associated with the manifestation of symptoms of endodontic origin. (AU)