Monocyte-derived dendritic cells from patients with severe forms of chromoblastomycosis induce CD4(+) T cell activation in vitro

Author(s):Show less -

Sousa, M. Gloria ^[1] ; Ghosn, E. Eid Bou ^[1] ; Nascimento, R. Ciciro ^[1] ; Bomfim, G. Facchioli ^[1] ; Noal, V. ^[1] ; Santiago, K. ^[1] ; Pedrozo e Silva Azevedo, C. de Maria ^[2] ; Marques, S. Garcia ^[2] ; Goncalves, A. Guedes ^[2] ; Lima Santos, D. Wagner de Castro ^[2] ; Criado, P. Ricardo ^[3] ; Martins, J. Eduardo Costa ^[3] ; Almeida, S. Rogerio ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Anal Clin & Toxicol, BR-05508900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Med, Dept Dermatol, BR-05508900 Sao Paulo - Brazil

^[3] Univ Fed Maranhao, Dept Patol, Sao Paulo - Brazil

• Document type: Journal article
• Source: CLINICAL AND EXPERIMENTAL IMMUNOLOGY; v. 156, n. 1, p. 117-125, APR 2009.
• Web of Science Citations: 3

Abstract

Dendritic cells (DCs) have been described as initiators and modulators of the immune response. Recently we have shown a predominant production of interleukin-10 cytokine, low levels of interferon-gamma and inefficient T cell proliferation in patients with severe forms of chromoblastomycosis. Chromoblastomycosis starts with subcutaneous inoculation of Fonsecaea pedrosoi into tissue where DCs are the first line of defence against this microorganism. In the present study, the interaction of F. pedrosoi and DCs obtained from patients with chromoblastomycosis was investigated. Our results showed that DCs from patients exhibited an increased expression of human leucocyte antigen D-related (HLA-DR) and co-stimulatory molecules. In the presence of conidia, the expression of HLA-DR and CD86 was up-regulated by DCs from patients and controls. Finally, we demonstrate the reversal of antigen-specific anergy and a T helper type 1 response mediated by DCs incubated with F. pedrosoi conidea. (AU)