| Full text | |
| Author(s): Show less - |
Menezes, Milene C.
[1]
;
Leme, Adriana F. Paes
[1]
;
Melo, Robson L.
[1]
;
Silva, Carlos A.
[1]
;
Della Casa, Maisa
[2]
;
Bruni, Fernanda M.
[1]
;
Lima, Carla
[1]
;
Lopes-Ferreira, Monica
[1]
;
Camargo, Antonio C. M.
[1]
;
Fox, Jay W.
[3]
;
Serrano, Solange M. T.
[1]
Total Authors: 11
|
| Affiliation: | [1] Inst Butantan, Lab Especial Toxinol Aplicada CAT CEPID, BR-05503900 Sao Paulo - Brazil
[2] Inst Butantan, Lab Imunopatol, BR-05503900 Sao Paulo - Brazil
[3] Univ Virginia, Dept Microbiol, Charlottesville, VA 22908 - USA
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | FEBS Letters; v. 582, n. 28, p. 3915-3921, NOV 26 2008. |
| Web of Science Citations: | 22 |
| Abstract | |
The functionality of the disintegrin-like/cysteine-rich domains of snake venom metalloproteinases (SVMPs) has been shown to reside in the cysteine-rich region, which can interact with VWA-containing proteins. Recently, the hyper-variable region (HVR) of the cysteine-rich domain was suggested to constitute a potential protein-protein adhesive interface. Here we show that recombinant proteins of HF3, a hemorrhagic P-III SVMP, containing the cysteine-rich domain (disintegrin-like/cysteinerich and cysteine-rich proteins) but not the disintegrin- like protein were able to significantly increase leukocyte rolling in the microcirculation. Peptides from the HVR also promoted leukocyte rolling and this activity was inhibited by anti-alpha(M)/beta(2) antibodies. These results show, for the first time, that the cysteine-rich domain and its HVR play a role in triggering pro-inflammatory effects mediated by integrins. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved. (AU) | |
| FAPESP's process: | 98/14307-9 - Center for Applied Toxinology |
| Grantee: | Hugo Aguirre Armelin |
| Support Opportunities: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |
| FAPESP's process: | 05/55821-2 - Papel do domínio rico em cisteínas do HF3, uma metaloproteinase do veneno de Bothrops jararaca, na inibição da agregação plaquetária e na ativação da fagocitose por macrófagos |
| Grantee: | Milene Cristina Menezes dos Santos |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| FAPESP's process: | 06/50059-8 - Proteômica aplicada ao estudo dos alvos celulares e plasmáticos do HF3, uma metaloproteinase do veneno da serpente Bothrops jararaca com atividades hemorrágica e inflamatória |
| Grantee: | Adriana Franco Paes Leme |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 04/15974-1 - Estudo sobre a função do domínio rico em cisteínas do fator hemorrágico 3 (HF3), metaloproteinases do veneno da serpente Bothrops jararaca, na interação com seus alvos celulares e plasmáticos |
| Grantee: | Solange Maria de Toledo Serrano |
| Support Opportunities: | Regular Research Grants |