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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Suramin affects metalloproteinase-9 activity and increases beta-dystroglycan levels in the diaphragm of the dystrophin-deficient mdx MOUSE

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Author(s):
Tiemi Taniguti, Ana Paula [1, 2] ; Matsumura, Cintia Yuri [2] ; Rodrigues-Simioni, Lea [1] ; Neto, Humberto Santo [2] ; Marques, Maria Julia [2]
Total Authors: 5
Affiliation:
[1] Univ Estadual Campinas UNICAMP, Fac Ciencias Med, Dept Farmacol, BR-13083970 Campinas, SP - Brazil
[2] Univ Estadual Campinas UNICAMP, Inst Biol, Dept Anat Biol Celular Fisiol & Biofis, BR-13083970 Campinas, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: MUSCLE & NERVE; v. 46, n. 5, p. 810-813, NOV 2012.
Web of Science Citations: 8
Abstract

Introduction: In Duchenne muscular dystrophy and in the mdx mouse, muscle fiber degeneration and subsequent fibrosis lead to cardiorespiratory failure. Previously, we demonstrated that the anti-fibrotic agent suramin was effective in decreasing fibrosis in mdx muscles. In this study, we were interested to see whether suramin could affect metalloproteinases (MMP) and improve the functional activity of the mdx diaphragm muscle. Methods: Zymography was performed to evaluate MMP-2 and MMP-9 activity. Western blotting was used to analyze the levels of beta-dystroglycan. Muscle function was assessed in hemidiaphragm in vitro preparations. Results: We found that suramin affects metalloproteinase-9 activity and increases beta-dystroglycan. Furthermore, suramin also protects against diaphragm muscle fatigue over time. Conclusions: These results show the potential benefits of suramin in maintaining the structure of the dystrophin-glycoprotein complex. Muscle Nerve, 46:810813, 2012 (AU)