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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Treatment with Azadirachta indica in diabetic pregnant rats: Negative effects on maternal outcome

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Author(s):
Dallaqua, Bruna [1] ; Saito, Felipe Hiroshi [1] ; Rodrigues, Tiago ; Paranhos Calderon, Iracema Mattos [1] ; Cunha Rudge, Marilza Vieira [1] ; Herrera, Emilio [2, 3] ; Damasceno, Debora Cristina [1]
Total Authors: 7
Affiliation:
[1] Univ Estadual Paulista UNESP, Botucatu Med Sch, Postgrad Program Gynecol, Lab Expt Res Gynecol & Obstet, Jaboticabal, SP - Brazil
[2] Univ San Pablo CEU, Fac Pharm, Madrid - Spain
[3] Univ San Pablo CEU, Fac Med, Madrid - Spain
Total Affiliations: 3
Document type: Journal article
Source: Journal of Ethnopharmacology; v. 143, n. 3, p. 805-811, OCT 11 2012.
Web of Science Citations: 18
Abstract

Ethnopharmacological relevance: The role of Azadirachta indica (neem) against Chagas disease and its antibiotic and antidiabetic action have been demonstrated in non-pregnant animals. However, the effects of neem on lipid metabolism and oxidative stress during pregnancy remain to be investigated. The objective of this study was to evaluate the effects of Azadirachta indica (neem) on maternal reproductive performance and biochemical parameters in non-diabetic and streptozotocin-induced mild diabetic rats (MD). Materials and methods: Pregnant rats were randomly distributed into six experimental groups: ND=non-treated non-diabetic (n=13); NDOil=non-diabetic treated with 1.2 mL/day neem seed oil (n=12); NDPA=non-diabetic treated with 1.0 mg/mL/day azadirachtin (n=12); D=non-treated diabetic (n=13); DOil: diabetic treated with neem seed oil (n=12), and DPA=diabetic treated with azadirachtin, n=13. Treatment with either neem oil (1.2 mL/day) or azadirachtin (1.0 mg/mL/day) was orally administered throughout pregnancy. Glucose test tolerance (GTT) was performed at day 17 of pregnancy and used as an inclusion criterion. At term pregnancy, maternal reproductive outcomes, lipid profile and oxidative stress status were assessed. Results: Treatment with neem oil and azadirachtin during pregnancy (1) had no hypoglycemic and anti-hyperglycemic effects on non-diabetic and diabetic rats, respectively; (2) affected OGTT glycemic levels in diabetic rats; (3) increased the proportion of fetuses classified as small for pregnancy age (SPA) in all groups; and (4) did not interfere with the lipid profile in non-diabetic dams. Neem oil reduced the rate of total cholesterol and NEFA in diabetic animals. Both neem oil and azadirachtin increased lipoperoxidation, characterized by increased MDA levels in non-diabetic rats. Conclusion: Both neem seed oil and azadirachtin impaired intrauterine development and altered antioxidant/oxidative status during pregnancy. (C) 2012 Elsevier Ireland Ltd. All rights reserved. (AU)

FAPESP's process: 09/14147-8 - Intervention using Azadirachta indica (Neem) in pregnancy of diabetic rats: maternal-fetal repercussions
Grantee:Débora Cristina Damasceno
Support Opportunities: Regular Research Grants