Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

TLR4 mRNA Levels as Tools to Estimate Risk for Early Posttransplantation Kidney Graft Dysfunction

Full text
Author(s):
Andrade-Oliveira, Vinicius [1, 2] ; Campos, Erika F. [1, 2] ; Goncalves-Primo, Amador [2] ; Grenzi, Patricia C. [1, 2] ; Medina-Pestana, Jose O. [1, 3] ; Tedesco-Silva, Helio [3] ; Gerbase-DeLima, Maria [1, 2]
Total Authors: 7
Affiliation:
[1] Univ Fed Sao Paulo, Sao Paulo - Brazil
[2] Assoc Fundo Incent Pesquisa, Inst Imunogenet, BR-04040031 Sao Paulo - Brazil
[3] Hosp Rim & Hipertensao, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: TRANSPLANTATION; v. 94, n. 6, p. 589-595, SEP 27 2012.
Web of Science Citations: 19
Abstract

Background. The participation of Toll-like receptor (TLR) 4, an innate immunity receptor, has been previously demonstrated in the pathogenesis of acute renal injury. We aimed to investigate whether messenger RNA (mRNA) levels of TLR4 and its adapter molecule, myeloid differentiation primary response gene (MYD) 88, are associated with delayed graft function (DGF) and could be used as biomarkers of its occurrence. Methods. TLR4 and MYD88 gene mRNA levels were evaluated with real-time polymerase chain reaction, in preimplantation biopsies (n=89) and first day posttransplantation samples of urine (n=67) and blood (n=80) from graft recipients and analyzed according to donor type (living or deceased) and DGF occurrence. Results. Expression levels of both genes were higher in biopsies from deceased donors than from living donors (P<0.001 for both) but did not differ between deceased-donor kidney transplants with and without DGF; in urine, TLR4 expression levels were higher in patients with prolonged DGF (DGF lasting 914 days) (P=0.05, compared with cases without DGF); in blood, lower mRNA levels of TLR4 and MYD88 predicted pDGF occurrence with an accuracy of 86% and 87%, respectively. Conclusion. The expression levels of TLR4 and MYD88 were higher in kidneys from deceased donors than from living donors. Lower levels of expression of both genes in blood were associated with DGF occurrence. The prediction of prolonged DGF by low TLR4 and MYD88 expression levels in blood with a greater the 85% accuracy was the most important finding of this study. (AU)