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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Molecular detection of tick-borne bacterial agents in Brazilian and exotic captive carnivores

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Author(s):
Andre, M. R. [1, 2] ; Dumler, J. S. [1] ; Scorpio, D. G. [1] ; Teixeira, R. H. F. [3] ; Allegretti, S. M. [4] ; Machado, R. Z. [2]
Total Authors: 6
Affiliation:
[1] Johns Hopkins Sch Med, Dept Pathol, Div Med Microbiol, Baltimore, MD 21205 - USA
[2] Univ Estadual Paulista UNESP, Lab Imunoparasitol, Dept Patol Vet, FCAV, BR-14884900 Jaboticabal, SP - Brazil
[3] Zool Sorocaba, BR-18020268 Sorocaba, SP - Brazil
[4] Univ Estadual Campinas Unicamp, Dept Parasitol, BR-13084971 Campinas, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: TICKS AND TICK-BORNE DISEASES; v. 3, n. 4, p. 247-253, 2012.
Web of Science Citations: 27
Abstract

The present study aims to detect and characterize by molecular techniques, the presence of tick-borne pathogens in wild captive carnivore blood samples from Brazil. Blood was collected from 76 Brazilian felids, 23 exotic felids, 3 European wolves (Canis lupus), and 97 Brazilian canids maintained in captivity in zoos located in Sao Paulo and Mato Grosso states, Brazil. DNA of each sample was used in PCR reactions for Ehrlichia, Anaplasma, and Rickettsia identification. The blood from 10/100(10%) of canids (1 European wolf, 3 bush dogs, and 6 crab-eating foxes) and from 21/99 (21%) felids (4 pumas, 6 little spotted cats. 4 ocelots, 3 jaguarundis, 1 tiger, and 3 lions) contained fragments of 16S rRNA gene of Ehrlichia spp. Fragments of Anaplasma spp. groESL and 16S rRNA genes were detected in the blood of 1/100(1%) canids (1 bush dog) and in 4/99(3%) felids (4 little spotted cats), respectively. Rickettsia species infections were not identified. The present work showed that new strains of Ehrlichia and Anaplasma spp. circulate among wild carnivores in Brazil. (c) 2012 Elsevier GmbH. All rights reserved. (AU)