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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Phylogenetic Analysis and DNA-based Species Confirmation in Anopheles (Nyssorhynchus)

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Author(s):
Foster, Peter G. [1] ; Bergo, Eduardo S. [2] ; Bourke, Brian P. [3] ; Oliveira, Tatiane M. P. [3] ; Nagaki, Sandra S. [3] ; Sant'Ana, Denise C. [3] ; Sallum, Maria Anice M. [3]
Total Authors: 7
Affiliation:
[1] Nat Hist Museum, Dept Life Sci, London SW7 5BD - England
[2] Secretaria Estado Saude Sao Paulo, Sao Paulo - Brazil
[3] Univ Sao Paulo, Fac Saude Publ, Dept Epidemiol, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: PLoS One; v. 8, n. 2 FEB 4 2013.
Web of Science Citations: 44
Abstract

Specimens of neotropical Anopheles (Nyssorhynchus) were collected and identified morphologically. We amplified three genes for phylogenetic analysis-the single copy nuclear white and CAD genes, and the COI barcode region. Since we had multiple specimens for most species we were able to test how well the single or combined genes were able to corroborate morphologically defined species by placing the species into exclusive groups. We found that single genes, including the COI barcode region, were poor at confirming species, but that the three genes combined were able to do so much better. This has implications for species identification, species delimitation, and species discovery, and we caution that single genes are not enough. Higher level groupings were partially resolved with some well-supported groupings, whereas others were found to be either polyphyletic or paraphyletic. There were examples of known groups, such as the Myzorhynchella Section, which were poorly supported with single genes but were well supported with combined genes. From this we can infer that more sequence data will be needed in order to show more higher-level groupings with good support. We got unambiguously good support (0.94-1.0 Bayesian posterior probability) from all DNA-based analyses for a grouping of An. dunhami with An. nuneztovari and An. goeldii, and because of this and because of morphological similarities we propose that An. dunhami be included in the Nuneztovari Complex. We obtained phylogenetic corroboration for new species which had been recognised by morphological differences; these will need to be formally described and named. (AU)

FAPESP's process: 11/20397-7 - Morphological and molecular taxonomy and phylogeny of Nyssorhynchus subgenus of Anopheles (Diptera: Culicidae), with special emphasis on Anopheles darlingi from Mata Atlântica
Grantee:Maria Anice Mureb Sallum
Support type: Regular Research Grants
FAPESP's process: 05/53973-0 - Systematics of the Anopheles (Nyssorhynchus) (Diptera: Culicidae)
Grantee:Maria Anice Mureb Sallum
Support type: Research Projects - Thematic Grants