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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Cigarette smoke exposure severely reduces peripheral insulin sensitivity without changing GLUT4 expression in oxidative muscle of Wistar rats

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Author(s):
Ebersbach-Silva, Patricia [1] ; Alves, Thamara [1] ; Sakata Fonseca, Agatha Tomoko [1] ; do Nascimento Oliveira, Marcia Aparecida [2] ; Machado, Ubiratan Fabres [2] ; Seraphim, Patricia Monteiro [1]
Total Authors: 6
Affiliation:
[1] Univ Estadual Paulista, Dept Phys Therapy, Sch Sci & Technol, UNESP, Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Arquivos Brasileiros de Endocrinologia e Metabologia; v. 57, n. 1, p. 19-26, FEB 2013.
Web of Science Citations: 4
Abstract

Objective: To evaluate the effect of exposure to cigarette smoke and running training on a treadmill on the expression of glucose transporter GLUT4 in oxidative soleus muscle of rats. Materials and methods: Wistar rats were divided into: (C) control, (E) exercise control, (SS), sedentary smoker, and (ES) exercise smoker. Insulin Tolerance Test, Western blotting, and RT-PCR were performed for the evaluation of GLUT4 levels. Results: The SS group presented lower insulin sensitivity with reduced GLUT4 protein in the plasma membrane (PM), no changes in the microsomal fraction, but increased mRNA content. Training reversed this condition. No intervention altered total GLUT4 content of the oxidative muscle. Conclusion: These results suggest that passive smoking stimulates GLUT4 transcription without changing total protein content, but impairs the ability of GLUT4 translocation to the PM. On the other hand, training seems to reduce the deleterious effects, even under the influence of cigarette smoking. Arq Bras Endocrinol Metab. 2013;57(1):19-26 (AU)

FAPESP's process: 08/09328-0 - GLUT4 glucose transporter expression evaluation in skeletal muscle soleum from sedentary and exercited passive smokers rats
Grantee:Ágatha Tomoko Sakata Fonseca
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 08/10886-8 - GLUT4 and SOCS-3 expression in skeletal muscle from smokers rats submitted to moderated aerobic exercise
Grantee:Thâmara Alves
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 08/01955-6 - Effect of passive tobaccoism and physical exercise-associated on glucose transporter GLUT4 expression in muscles of rats
Grantee:Patrícia Ebersbach Silva
Support type: Scholarships in Brazil - Master