Effects of Antimicrobial Photodynamic Therapy on Transforming Growth Factor-beta 1 Levels in the Gingival Crevicular Fluid

Author(s)

Souza, Sergio L. S. ^[1] ; Andrade, Patricia F. ^[1] ; Silva, Joao S. ^[2] ; Tristao, Fabrine S. M. ^[2] ; Rocha, Fernanda A. ^[2] ; Palioto, Daniela B. ^[1] ; Grisi, Marcio F. M. ^[1] ; Taba, Jr., Mario ^[1] ; Novaes, Jr., Arthur B. ^[1]

Affiliation

^[1] Univ Sao Paulo, Dept Buccomaxillofacial Surg & Traumatol & Period, Sch Dent Ribeirao Preto, BR-14040904 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Dept Immunol, Sch Med Ribeirao Preto, BR-14040904 Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: Photomedicine and Laser Surgery; v. 31, n. 2, p. 65-71, FEB 2013.
• Web of Science Citations: 6

Abstract

Background and objective: Antimicrobial photodynamic therapy (aPDT) has been proposed as an adjunctive therapy to scaling and root planing (SRP). The transforming growth factor-beta 1 (TGF-beta) has been considered as an anti-inflammatory cytokine, and its levels in the gingival crevicular fluid (GCF) could monitor the periodontal repair. This study evaluated the adjunct effect of aPDT compared with SRP, analyzing the TGF-beta levels in GCF after nonsurgical and surgical therapy in chronic periodontitis patients. Methods: Fifteen patients, presenting bilaterally lower molars with class III furcation lesions, were selected. Each pair of teeth was randomly assigned to a control group (CG) or test group (TG). Initially, SRP was performed in the CG, and SRP + aPDT in the TG. Forty-five days later, flap surgery plus SRP, and flap surgery plus SRP + aPDT were performed in CG and TG, respectively. GCF was collected and an enzyme-linked immunosorbent assay (ELISA) test was conducted to determine the amount and concentration of TGF-beta in the GCF at baseline, 45 days post-initial therapy, and 21 days after surgery. Results: Statistically significant differences between groups were found in relation to GCF volume 21 days after the surgical procedures (p = 0.03) and TGF-beta concentration in GCF 45 days post-initial therapy (p = 0.04), favoring the TG. Conclusions: There was an additional effect of the aPDT protocol compared with SRP for the TGF-beta concentration in GCF 45 days after nonsurgical therapy, and for the GCF volume 21 days after surgical therapy. (AU)

• FAPESP's process: 07/04916-9 - Evaluation of the photodynamic therapy on root decontamination and modulation of the immuno-inflammatory response and healing of the periodontal tissues: a randomized controlled clinical study in humans
• Grantee: Sérgio Luís Scombatti de Souza
• Support Opportunities: Regular Research Grants