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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Molecular Characterization of Wolbachia Strains Associated with the Invasive Asian Citrus Psyllid Diaphorina citri in Brazil

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Author(s):
Guidolin, A. S. [1] ; Consoli, F. L. [1, 2]
Total Authors: 2
Affiliation:
[1] Univ Sao Paulo, ESALQ, Dept Entomol & Acarol, Insect Interact Lab, BR-13418900 Sao Paulo - Brazil
[2] Univ Sao Paulo, ESALQ, Dept Entomol & Acarol, BR-13418900 Piracicaba, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: MICROBIAL ECOLOGY; v. 65, n. 2, p. 475-486, FEB 2013.
Web of Science Citations: 19
Abstract

Wolbachia is a symbiont intensively studied due to its ability to interfere with their host's reproduction, and it has been recently proposed as an alternative tool to control insect pests or vectors of diseases. The Asian citrus psyllid Diaphorina citri is an important pest of citrus since it vectors the bacterium that causes the ``Huanglongbing{''} disease in citrus. The frequency and diversity of Wolbachia associated with D. citri is unknown, limiting the utilization of Wolbachia as an alternative strategy for insect management. Thus, we aimed to determine the natural rate of infection, to characterize the Wolbachia strains associated with this psyllid by ``multilocus sequencing typing{''} (MLST) and wsp analysis, and to verify the association of the symbiont to particular genotypes of the host. Analysis indicated Wolbachia infects 100 % of all specimens tested from all 15 sampled populations. MLST revealed the occurrence of five new sequence types (STs) of Wolbachia, while analysis based on the wsp sequences indicated only four different types of Wolbachia. ST-173 was predominant, while the remaining STs were population specific. Analysis of the host-symbiont relationship did not reveal any particular association of Wolbachia and haplotypes or a decrease in nucleotide diversity of D. citri in populations in which more than one ST was recorded. The consequences of the diversity of STs reported are still unknown, but the fact that Wolbachia infection is fixed and that there is one ST with a broad distribution highlights the use of this symbiont as an alternative strategy to control D. citri. (AU)

FAPESP's process: 04/14215-0 - Bioecology and development of control strategies of Diaphorina citri Kuwayama (Hemiptera: Psyllidae), vector of the greening-causing bacterium
Grantee:José Roberto Postali Parra
Support Opportunities: Research Projects - Thematic Grants