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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Glytube: A Conical Tube and Parafilm M-Based Method as a Simplified Device to Artificially Blood-Feed the Dengue Vector Mosquito, Aedes aegypti

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Author(s):
Costa-da-Silva, Andre Luis [1] ; Navarrete, Flavia Rosa [1] ; Salvador, Felipe Scassi [2] ; Karina-Costa, Maria [1] ; Ioshino, Rafaella Sayuri [1] ; Azevedo, Diego Soares [1] ; Rocha, Desiree Rafaela [1] ; Romano, Camila Malta [2] ; Capurro, Margareth Lara [1]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Parasitol, BR-05508 Sao Paulo - Brazil
[2] Univ Sao Paulo, Virol Lab, Inst Trop Med, Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: PLoS One; v. 8, n. 1 JAN 14 2013.
Web of Science Citations: 14
Abstract

Aedes aegypti, the main vector of dengue virus, requires a blood meal to produce eggs. Although live animals are still the main blood source for laboratory colonies, many artificial feeders are available. These feeders are also the best method for experimental oral infection of Ae. aegypti with Dengue viruses. However, most of them are expensive or laborious to construct. Based on principle of Rutledge-type feeder, a conventional conical tube, glycerol and Parafilm-M were used to develop a simple in-house feeder device. The blood feeding efficiency of this apparatus was compared to a live blood source, mice, and no significant differences (p = 0.1189) were observed between artificial-fed (51.3% of engorgement) and mice-fed groups (40.6%). Thus, an easy to assemble and cost-effective artificial feeder, designated ``Glytube{''} was developed in this report. This simple and efficient feeding device can be built with common laboratory materials for research on Ae. aegypti. (AU)

FAPESP's process: 09/53637-0 - Anopheles-Plasmodium interaction: a multidisciplinary approach
Grantee:Margareth de Lara Capurro-Guimarães
Support type: Research Projects - Thematic Grants
FAPESP's process: 08/10254-1 - Designing of a virus-regulated mosquito lethal gene
Grantee:Margareth de Lara Capurro-Guimarães
Support type: Regular Research Grants