A Preliminary and Qualitative Metallomics Study of Mercury in the Muscle of Fish from Amazonas, Brazil

Author(s)

Moraes, Paula M. ^[1] ; Santos, Felipe A. ^[1] ; Padilha, Cilene C. F. ^[1] ; Vieira, Jose C. S. ^[1] ; Zara, Luiz F. ^[2] ; Padilha, Pedro de M. ^{[1, 3]}

Affiliation

^[1] UNESP, Inst Biociencias, Botucatu, SP - Brazil

^[2] Univ Brasilia UNB, Brasilia, DF - Brazil

^[3] Univ Estadual Campinas, Inst Nacl Ciencia & Tecnol Bioanalit, Campinas, SP - Brazil

• Document type: Journal article
• Source: BIOLOGICAL TRACE ELEMENT RESEARCH; v. 150, n. 1-3, p. 195-199, DEC 2012.
• Web of Science Citations: 12

Abstract

This paper presents preliminary findings for a metallomics study of mercury in the muscle of the fish species from Amazonas, Brazil, after protein separation by two-dimensional polyacrylamide gel electrophoresis and subsequent evaluation of mercury by synchrotron radiation X-ray fluorescence. The fluorescence spectra revealed mercury in two protein spots. The mercury-containing protein spots showed molecular weights of 20.8 +/- 0.7 and 19.8 +/- 0.5 kDa and isoelectric points of 5.6 +/- 0.2 and 7.5 +/- 0.3, respectively. (AU)

• FAPESP's process: 09/54856-8 - Development of analytical methods for mercury metallomics studies in fish collected in the AHE JIRAU - Madeira River Basin area of influence
• Grantee: Paula Martin de Moraes
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 10/51332-5 - Development of analytical methods for mercury metallomics studies in fish collected in the AHE JIRAU - Madeira River Basin area of influence
• Grantee: Pedro de Magalhães Padilha
• Support Opportunities: Regular Research Grants