| Full text | |
| Author(s): |
Barbosa Chaves, Karen Cristina
[1]
;
Turaca, Lauro Thiago
[2]
;
Pesquero, Joao Bosco
[2]
;
Mennecier, Gregory
[3]
;
Zaidan Dagli, Maria Lucia
[3]
;
Chammas, Roger
[4]
;
Schor, Nestor
[1]
;
Bellini, Maria Helena
[1, 5]
Total Authors: 8
|
| Affiliation: | [1] Univ Fed Sao Paulo, Dept Med, Div Nephrol, Sao Paulo - Brazil
[2] Univ Fed Sao Paulo, Dept Biophys, Sao Paulo - Brazil
[3] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Pathol, Sao Paulo - Brazil
[4] Univ Sao Paulo, Dept Radiol, Sao Paulo - Brazil
[5] Univ Sao Paulo, IPEN CNEN, Dept Biotechnol, Sao Paulo - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | BIOMEDICINE & PHARMACOTHERAPY; v. 66, n. 6, p. 464-468, SEP 2012. |
| Web of Science Citations: | 9 |
| Abstract | |
Tumor cells induce the disruption of homeostasis between cellular and extracellular compartments to favor tumor progression. The expression of fibronectin (FN), a matrix glycoprotein, is increased in several carcinoma cell types, including renal cell carcinoma (RCC). RCC are highly vascularized tumors and are often amenable to antiangiogenic therapy. Endostatin (ES) is a fragment of collagen XVIII that possesses antiangiogenic activity. In this study, we examined the modulation of FN gene expression by ES gene therapy in a murine metastatic renal cell carcinoma (mRCC) model. Balb/C mice bearing Renca cells were treated with NIH/3T3-LXSN cells or NIH/3T3-LendSN cells. At the end of the experiment, the ES serum levels were measured, and the FN gene expression was assessed using real-time PCR. The tissue FN was evaluated by western blotting and by immunofluorescence analysis. The ES serum levels in treated mice were higher than those in the control group (P < 0.05). ES treatment led to significant decreases at the FN mRNA (P < 0.001) and protein levels (P < 0.01). Here, we demonstrate the ES antitumor effect that is mediated by down-regulation of FN expression in mRCC. (C) 2012 Elsevier Masson SAS. All rights reserved. (AU) | |
| FAPESP's process: | 09/12518-9 - Evaluation of expression of adhesion molecules (VCAM and ICAM) in renal metastatic lung carcinoma of animals submitted to gene therapy with endostatin |
| Grantee: | Karen Cristina Barbosa Chaves |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 10/18969-0 - NF-kB regulation in the renal cell carcinoma after endostatin gene therapy |
| Grantee: | Maria Helena Bellini Marumo |
| Support Opportunities: | Regular Research Grants |