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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Biosynthetic origins of the isoprene units of 4-nerolidylcatechol in Potomorphe umbellata

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Author(s):
Debora Cristina B. Bergamo [1] ; Massuo Jorge Kato [2] ; Vanderlan da S. Bolzani [3] ; Maysa Furlan [4]
Total Authors: 4
Affiliation:
[1] Universidade Estadual Paulista. Instituto de Química - Brasil
[2] Universidade de São Paulo. Instituto de Química - Brasil
[3] Universidade Estadual Paulista. Instituto de Química - Brasil
[4] Universidade Estadual Paulista. Instituto de Química - Brasil
Total Affiliations: 4
Document type: Journal article
Source: Journal of the Brazilian Chemical Society; v. 16, p. 1406-1409, 2005-11-00.
Field of knowledge: Physical Sciences and Mathematics - Chemistry
Abstract

The biosynthetic origins of the isoprene units of 4-nerolidylcatechol (1), the major constituent of Potomorphe umbellata, have been studied through feeding experiments with [14C]- and [13C]-glucose, and with precursors of the mevalonic acid and triose/pyruvate pathways, namely, [2-14C]-mevalonolactone and [U-14C]-glyceraldehyde-3-phosphate, respectively. The pattern of incorporation of label from [1-13C]-glucose into 1 was determined by quantitative 13C NMR spectroscopy. The labelling pattern revealed that the additive was specifically incorporated, and that the isoprene units of the sesquiterpenoid moiety of 4-nerolidylcatechol were derived from both the mevalonic acid and the triose/pyruvate pathways. The results indicate that both plastidic and cytoplasmic pathways are able to provide isopentenyl diphosphate units for the biosynthesis of 1. (AU)