| Full text | |
| Author(s): |
Debora Cristina B. Bergamo
[1]
;
Massuo Jorge Kato
[2]
;
Vanderlan da S. Bolzani
[3]
;
Maysa Furlan
[4]
Total Authors: 4
|
| Affiliation: | [1] Universidade Estadual Paulista. Instituto de Química - Brasil
[2] Universidade de São Paulo. Instituto de Química - Brasil
[3] Universidade Estadual Paulista. Instituto de Química - Brasil
[4] Universidade Estadual Paulista. Instituto de Química - Brasil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Journal of the Brazilian Chemical Society; v. 16, p. 1406-1409, 2005-11-00. |
| Field of knowledge: | Physical Sciences and Mathematics - Chemistry |
| Abstract | |
The biosynthetic origins of the isoprene units of 4-nerolidylcatechol (1), the major constituent of Potomorphe umbellata, have been studied through feeding experiments with [14C]- and [13C]-glucose, and with precursors of the mevalonic acid and triose/pyruvate pathways, namely, [2-14C]-mevalonolactone and [U-14C]-glyceraldehyde-3-phosphate, respectively. The pattern of incorporation of label from [1-13C]-glucose into 1 was determined by quantitative 13C NMR spectroscopy. The labelling pattern revealed that the additive was specifically incorporated, and that the isoprene units of the sesquiterpenoid moiety of 4-nerolidylcatechol were derived from both the mevalonic acid and the triose/pyruvate pathways. The results indicate that both plastidic and cytoplasmic pathways are able to provide isopentenyl diphosphate units for the biosynthesis of 1. (AU) | |