Localização de transcritos de Bmp-4, Shh e Wnt-5a durante as fases iniciais do desenvolvimento dentário de camundongos por hibridização in situ

Fábio Daumas Nunes; Maria da Graça Silva Valenzuela; Camila Oliveira Rodini; Silvia Maria Gomes Massironi; Gui Mi Ko

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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Localization of Bmp-4, Shh and Wnt-5a transcripts during early mice tooth development by in situ hybridization

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Author(s):	Fábio Daumas Nunes ; Maria da Graça Silva Valenzuela ; Camila Oliveira Rodini ^[3] ; Silvia Maria Gomes Massironi ^[4] ; Gui Mi Ko ^[5] Total Authors: 5
Document type:	Journal article
Source:	Brazilian Oral Research; v. 21, n. 2, p. 127-133, 2007-06-00.
Abstract
A comparative nonisotopic in situ hybridization (ISH) analysis was carried out for the detection of Bmp-4, Shh and Wnt-5a transcripts during mice odontogenesis from initiation to cap stage. Bmp-4 was expressed early in the epithelium and then in the underlying mesenchyme. Shh expression was seen in the odontogenic epithelial lining thickening, being stronger in the enamel knot area, during the cap stage. Wnt-5a transcripts were expressed only in the mesenchyme during the initiation, bud and cap stages, with strong expression in the dental mesenchyme during the bud stage. The present results showed that Bmp-4, Shh and Wnt-5a are expressed since the very early stages of tooth development, and they suggest that the Wnt-5a gene is expressed in different cell populations than Bmp-4 and Shh. (AU)

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