Evaluation of the cytocompatibility of mixed bovine bone

Author(s)

Esther Rieko Takamori ; Eduardo Aleixo Figueira ; Rumio Taga ; Mari Cleide Sogayar ; José Mauro Granjeiro

• Document type: Journal article
• Source: Brazilian Dental Journal; v. 18, n. 3, p. 179-184, 2007.

Abstract

Treatment of bovine bone with peroxides and chaotropic agents aims to obtain an acellular bone matrix that is able to maintain the collagen-apatite complex and a higher mechanical resistance, a mixed biomaterial hereby named mixed bovine bone (MBB). The purpose of this study was to evaluate the cytocompatibility of MBB and cell-MBB interaction. Cell morphology, number of viable cells, ability to reduce methyltetrazolium and to incorporate neutral red upon exposure to different concentrations of the hydrosoluble extract of MBB were assessed in Balb-c 3T3 cells according to ISO 10993-5 standard. The interaction between cells and MBB surface was evaluated by scanning electron microscopy. The water-soluble MBB extracts were cytotoxic and led to cell death possibly due to its effect on mitochondrial function and membrane permeability. Cells plated directly onto the MBB did not survive, although after dialysis and material conditioning in DMEM + 10% FCS, the cells adhered and proliferated onto the material. It may be concluded that, in vitro, water-soluble MBB extracts were cytotoxic. Nevertheless, MBB cytotoxic effect was reverted by dialysis resulting in a material that is suitable for cell based-therapy in the bioengineering field. (AU)

• FAPESP's process: 01/10707-7 - Molecular bases of the control of cell proliferation and origin of neoplasms in the genomic and proteomic era
• Grantee: Mari Cleide Sogayar
• Support type: Research Projects - Thematic Grants
• FAPESP's process: 99/06460-4 - Microencapsulation of human pancreatic islets and tissue engineering as an alternative therapy of Diabetes mellitus
• Grantee: Mari Cleide Sogayar
• Support type: Research Grants - Research Partnership for Technological Innovation - PITE