| Full text | |
| Author(s): |
Priolli, Denise G.
[1]
;
Canelloi, Thamy P.
[2]
;
Lopes, Camila O.
[2]
;
Valdivia, Julio C. M.
[2]
;
Martinez, Natalia P.
[2]
;
Acari, Demetrius P.
[2]
;
Cardinalli, Izilda A.
[3]
;
Ribeiro, Marcelo L.
[1]
Total Authors: 8
|
| Affiliation: | [1] Univ Sao Francisco, Sch Med, Postgrad Program Strictu Senso Hlth Sci, Sao Paulo - Brazil
[2] Univ Sao Francisco, Sch Med, Sao Paulo - Brazil
[3] Univ Sao Francisco, Sch Med, Dept Pathol, Sao Paulo - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | INTERNATIONAL JOURNAL OF COLORECTAL DISEASE; v. 28, n. 5, p. 713-722, MAY 2013. |
| Web of Science Citations: | 4 |
| Abstract | |
Oxidative DNA damage is one of the mechanisms associated to initial colorectal carcinogenesis, but how it interacts with beta-catenin, an adherence protein related to cancer evolution, is not clear. This study investigates the relationship between oxidative DNA damage and beta-catenin expression in normal mucosa and colon tumor tissue (adenoma and adenocarcinoma) in colorectal adenocarcinoma evolution. One hundred and 13 samples were studied. Hematoxylin-eosin determined histological grade. beta-Catenin expression was analyzed by immunohistochemistry. The oxidative DNA damage was evaluated using comet assay technique. The coefficient for rejection of the nullity hypothesis was taken to 5 %. Kruskal-Wallis, Spearman test, and partial correlation were used to analyze the data. There was oxidative DNA damage increase in colorectal cancer evolution (p < 0.01). Histological grade was correlated with oxidative DNA damage (p < 0.01). There were differences in beta-catenin expression among normal, adenoma, and adenocarcinoma tissue with progressive increase of beta-catenin expression (p < 0.00). Histological grade was correlated to beta-catenin expression (p < 0.00). There was a relationship (p < 0.00) between beta-catenin and histological grade while controlling for the effect of oxidative DNA damage. The findings of this study make it possible to establish a relationship between oxidative DNA damage and beta-catenin expression in normal mucosa and colorectal tumor tissue. Additionally, they show a causal relationship between variations of beta-catenin in different tissues analyzed while controlling for the effect of oxidative DNA damage. (AU) | |
| FAPESP's process: | 07/01196-5 - Relationship between oxidative stress and morphofunctional classes of colorectal carcinoma, assessment by expression of cellular adhesion protein (CEA, E-cadherin, beta-cathenin) and carcinoembryonic antigen dynamics |
| Grantee: | Denise Gonçalves Priolli |
| Support type: | Regular Research Grants |