Oxidative DNA damage and beta-catenin expression in colorectal cancer evolution

Author(s)

Priolli, Denise G. ^[1] ; Canelloi, Thamy P. ^[2] ; Lopes, Camila O. ^[2] ; Valdivia, Julio C. M. ^[2] ; Martinez, Natalia P. ^[2] ; Acari, Demetrius P. ^[2] ; Cardinalli, Izilda A. ^[3] ; Ribeiro, Marcelo L. ^[1]

Affiliation

^[1] Univ Sao Francisco, Sch Med, Postgrad Program Strictu Senso Hlth Sci, Sao Paulo - Brazil

^[2] Univ Sao Francisco, Sch Med, Sao Paulo - Brazil

^[3] Univ Sao Francisco, Sch Med, Dept Pathol, Sao Paulo - Brazil

• Document type: Journal article
• Source: INTERNATIONAL JOURNAL OF COLORECTAL DISEASE; v. 28, n. 5, p. 713-722, MAY 2013.
• Web of Science Citations: 4

Abstract

Oxidative DNA damage is one of the mechanisms associated to initial colorectal carcinogenesis, but how it interacts with beta-catenin, an adherence protein related to cancer evolution, is not clear. This study investigates the relationship between oxidative DNA damage and beta-catenin expression in normal mucosa and colon tumor tissue (adenoma and adenocarcinoma) in colorectal adenocarcinoma evolution. One hundred and 13 samples were studied. Hematoxylin-eosin determined histological grade. beta-Catenin expression was analyzed by immunohistochemistry. The oxidative DNA damage was evaluated using comet assay technique. The coefficient for rejection of the nullity hypothesis was taken to 5 %. Kruskal-Wallis, Spearman test, and partial correlation were used to analyze the data. There was oxidative DNA damage increase in colorectal cancer evolution (p < 0.01). Histological grade was correlated with oxidative DNA damage (p < 0.01). There were differences in beta-catenin expression among normal, adenoma, and adenocarcinoma tissue with progressive increase of beta-catenin expression (p < 0.00). Histological grade was correlated to beta-catenin expression (p < 0.00). There was a relationship (p < 0.00) between beta-catenin and histological grade while controlling for the effect of oxidative DNA damage. The findings of this study make it possible to establish a relationship between oxidative DNA damage and beta-catenin expression in normal mucosa and colorectal tumor tissue. Additionally, they show a causal relationship between variations of beta-catenin in different tissues analyzed while controlling for the effect of oxidative DNA damage. (AU)

• FAPESP's process: 07/01196-5 - Relationship between oxidative stress and morphofunctional classes of colorectal carcinoma, assessment by expression of cellular adhesion protein (CEA, E-cadherin, beta-cathenin) and carcinoembryonic antigen dynamics
• Grantee: Denise Gonçalves Priolli
• Support Opportunities: Regular Research Grants