| Full text | |
| Author(s): Show less - |
Machado Motta, Maria Cristina
[1]
;
de Azevedo Martins, Allan Cezar
[1]
;
de Souza, Silvana Sant'Anna
[1, 2]
;
Costa Catta-Preta, Carolina Moura
[1]
;
Silva, Rosane
[2]
;
Klein, Cecilia Coimbra
[3, 4, 5]
;
Paula de Almeida, Luiz Gonzaga
[3]
;
Cunha, Oberdan de Lima
[3]
;
Ciapina, Luciane Prioli
[3]
;
Brocchi, Marcelo
[6]
;
Colabardini, Ana Cristina
[7]
;
Lima, Bruna de Araujo
[6]
;
Machado, Carlos Renato
[8]
;
de Almeida Soares, Celia Maria
[9]
;
Probst, Christian Macagnan
[10, 11]
;
Afonso de Menezes, Claudia Beatriz
[12]
;
Thompson, Claudia Elizabeth
[3]
;
Bartholomeu, Daniella Castanheira
[13]
;
Gradia, Daniela Fiori
[11]
;
Pavoni, Daniela Parada
[10]
;
Grisard, Edmundo C.
[14]
;
Fantinatti-Garboggini, Fabiana
[12]
;
Marchini, Fabricio Klerynton
[10]
;
Rodrigues-Luiz, Gabriela Flaia
[13]
;
Wagner, Glauber
[14]
;
Goldman, Gustavo Henrique
[7]
;
Rangel Fietto, Juliana Lopes
[15]
;
Elias, Maria Carolina
[16]
;
Goldman, Maria Helena S.
[17]
;
Sagot, Marie-France
[4, 5]
;
Pereira, Maristela
[9]
;
Stoco, Patricia H.
[14]
;
de Mendonca-Neto, Rondon Pessoa
[8]
;
Ribeiro Teixeira, Santuza Maria
[8]
;
Ferreira Maciel, Talles Eduardo
[15]
;
de Oliveira Mendes, Tiago Antonio
[13]
;
Uermenyi, Turan P.
[2]
;
de Souza, Wanderley
[1]
;
Schenkman, Sergio
[18]
;
Ribeiro de Vasconcelos, Ana Tereza
[3]
Total Authors: 40
|
| Affiliation: Show less - | [1] Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Lab Ultraestrutura Celular Hertha Meyer, BR-21941 Rio De Janeiro - Brazil
[2] Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Lab Metab Macromol Firmino Torres de Castro, BR-21941 Rio De Janeiro - Brazil
[3] Lab Bioinformat, Lab Nacl Computacao Cient, Rio De Janeiro - Brazil
[4] INRIA Grenoble Rhone Alpes, BAMBOO Team, Villeurbanne - France
[5] Univ Lyon 1, CNRS, UMR5558, Lab Biometrie & Biol Evolut, F-69622 Villeurbanne - France
[6] Univ Estadual Campinas, Inst Biol, Dept Genet Evolucao & Bioagentes, Sao Paulo - Brazil
[7] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Ciencias Farmaceut, Sao Paulo - Brazil
[8] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Bioquim & Imunol, Belo Horizonte, MG - Brazil
[9] Univ Fed Goias, Inst Ciencias Biol, Mol Biol Lab, Goiania, Go - Brazil
[10] Fundacao Oswaldo Cruz, Inst Carlos Chagas, Lab Genom Func, Curitiba, Parana - Brazil
[11] Fundacao Oswaldo Cruz, Inst Carlos Chagas, Lab Biol Mol Tripanossomatideos, Curitiba, Parana - Brazil
[12] Univ Estadual Campinas, Ctr Pluridisciplinar Pesquisas Quim Biol & Agr, Sao Paulo - Brazil
[13] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Parasitol, Belo Horizonte, MG - Brazil
[14] Univ Fed Santa Catarina, Dept Microbiol Imunol & Parasitol, Ctr Ciencias Biol, Lab Protozool & Bioinformat, Florianopolis, SC - Brazil
[15] Univ Fed Vicosa, Dept Bioquim & Biol Mol, Ctr Ciencias Biol & Saude, Vicosa, MG - Brazil
[16] Inst Butantan, Lab Especial Ciclo Celular, Sao Paulo - Brazil
[17] Univ Sao Paulo, Dept Biol, Fac Filosofia Ciencias & Letras Ribeirao Preto, Sao Paulo - Brazil
[18] Univ Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, Sao Paulo - Brazil
Total Affiliations: 18
|
| Document type: | Journal article |
| Source: | PLoS One; v. 8, n. 4 APR 3 2013. |
| Web of Science Citations: | 32 |
| Abstract | |
Endosymbiont-bearing trypanosomatids have been considered excellent models for the study of cell evolution because the host protozoan co-evolves with an intracellular bacterium in a mutualistic relationship. Such protozoa inhabit a single invertebrate host during their entire life cycle and exhibit special characteristics that group them in a particular phylogenetic cluster of the Trypanosomatidae family, thus classified as monoxenics. In an effort to better understand such symbiotic association, we used DNA pyrosequencing and a reference-guided assembly to generate reads that predicted 16,960 and 12,162 open reading frames (ORFs) in two symbiont-bearing trypanosomatids, Angomonas deanei (previously named as Crithidia deanei) and Strigomonas culicis (first known as Blastocrithidia culicis), respectively. Identification of each ORF was based primarily on TriTrypDB using tblastn, and each ORF was confirmed by employing getorf from EMBOSS and Newbler 2.6 when necessary. The monoxenic organisms revealed conserved housekeeping functions when compared to other trypanosomatids, especially compared with Leishmania major. However, major differences were found in ORFs corresponding to the cytoskeleton, the kinetoplast, and the paraflagellar structure. The monoxenic organisms also contain a large number of genes for cytosolic calpain-like and surface gp63 metalloproteases and a reduced number of compartmentalized cysteine proteases in comparison to other TriTryp organisms, reflecting adaptations to the presence of the symbiont. The assembled bacterial endosymbiont sequences exhibit a high A+T content with a total of 787 and 769 ORFs for the Angomonas deanei and Strigomonas culicis endosymbionts, respectively, and indicate that these organisms hold a common ancestor related to the Alcaligenaceae family. Importantly, both symbionts contain enzymes that complement essential host cell biosynthetic pathways, such as those for amino acid, lipid and purine/pyrimidine metabolism. These findings increase our understanding of the intricate symbiotic relationship between the bacterium and the trypanosomatid host and provide clues to better understand eukaryotic cell evolution. (AU) | |