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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The ovarian cycle histochemistry and its relationship with hepatopancreas weight in the blue crab Callinectes danae (Crustacea: Portunidae)

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Author(s):
Zara, Fernando J. [1, 2] ; Gaeta, Henrique H. [3] ; Costa, Tania M. [3] ; Toyama, Marcos H. [3] ; Caetano, Flavio H. [4]
Total Authors: 5
Affiliation:
[1] CAUNESP, BR-14884900 Jaboticabal, SP - Brazil
[2] Univ Estadual Paulista UNESP, Dept Biol Aplicada, Lab Morfol Invertebrados, FCAV, BR-14884900 Jaboticabal, SP - Brazil
[3] Univ Estadual Paulista UNESP, BR-11330900 Sao Vicente, SP - Brazil
[4] Univ Estadual Paulista UNESP, Dept Biol, BR-13506900 Rio Claro, SP - Brazil
Total Affiliations: 4
Document type: Review article
Source: ACTA ZOOLOGICA; v. 94, n. 2, p. 134-146, APR 2013.
Web of Science Citations: 9
Abstract

Zara, F.J., Gaeta, H.H., Costa, T.M., Toyama, M.H. and Caetano, F.H. 2011. The ovarian cycle histochemistry and its relationship with hepatopancreas weight in the blue crab Callinectes danae (Crustacea: Portunidae). Acta Zoologica (Stockholm) 00:113. Several studies use macroscopic patterns of the ovarian development in crustaceans. Here, we examined the relationship between ovary histochemistry, changes in gonadosomatic and hepatosomatic indices against the macroscopic pattern of the ovarian development in Callinectes danae. Animals were collected in the south coast of SAo Paulo State, Brazil. Ovaries were macroscopically classified as juvenile, rudimentary, developing, intermediary, mature, and rudimentary ovigerous. Samples were fixed in 4% paraformaldehyde, processed for historesin, and stained with HE, protein, and neutral and acid polysaccharides detection. The juvenile oocytes are not enclosed by follicular cells and have fewer yolk nuclei being less intense in PAS reactivity than rudimentary oocytes. Developing oocytes show yolk granules and thick follicular cells. Yolk granules were positive for proteins and neutral polysaccharides. The intermediary stage is marked by a qualitative increase in yolk granules and the onset of chorion formation. In mature oocytes, cytoplasm is completely filled by yolk granules and the chorion is completely formed. Ovigerous ovaries have several atretic follicles and large quantities of hemocytes in the process of tissue reorganization. In C.danae, the changes in cell, goandosomatic and hepatosomatic indices coinciding with macroscopic observations and any combination of different macroscopic stages in a single pattern should be avoided. (AU)