| Full text | |
| Author(s): Show less - |
Duarte, Margarida D.
[1]
;
Henriques, Ana Margarida
[1]
;
Barros, Silvia Carla
[1]
;
Fagulha, Teresa
[1]
;
Mendonca, Paula
[1]
;
Carvalho, Paulo
[1]
;
Monteiro, Madalena
[1]
;
Fevereiro, Miguel
[1]
;
Basto, Mafalda P.
[2]
;
Rosalino, Luis Miguel
[2, 3]
;
Barros, Tania
[4, 5]
;
Bandeira, Victor
[4, 5]
;
Fonseca, Carlos
[4, 5]
;
Cunha, Monica V.
[1]
Total Authors: 14
|
| Affiliation: | [1] IP Inst Nacl Invest Agr & Vet, INIAV, Unidade Estrateg Prod & Saude Anim, Lisbon - Portugal
[2] Univ Lisbon, Ctr Biol Ambiental, Fac Ciencias Lisboa, P-1699 Lisbon - Portugal
[3] Univ Sao Paulo, Lab Ecol Isotop CENA, Sao Paulo - Brazil
[4] Univ Aveiro, Dept Biol, P-3800 Aveiro - Portugal
[5] Univ Aveiro, CESAM, P-3800 Aveiro - Portugal
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | PLoS One; v. 8, n. 3 MAR 20 2013. |
| Web of Science Citations: | 22 |
| Abstract | |
The exposure of wild carnivores to viral pathogens, with emphasis on parvovirus (CPV/FPLV), was assessed based on the molecular screening of tissue samples from 128 hunted or accidentally road- killed animals collected in Portugal from 2008 to 2011, including Egyptian mongoose (Herpestes ichneumon, n = 99), red fox (Vulpes vulpes, n = 19), stone marten (Martes foina, n = 3), common genet (Genetta genetta, n = 3) and Eurasian badger (Meles meles, n = 4). A high prevalence of parvovirus DNA (63%) was detected among all surveyed species, particularly in mongooses (58%) and red foxes (79%), along with the presence of CPV/FPLV circulating antibodies that were identified in 90% of a subset of parvovirus- DNA positive samples. Most specimens were extensively autolysed, restricting macro and microscopic investigations for lesion evaluation. Whenever possible to examine, signs of active disease were not present, supporting the hypothesis that the parvovirus vp2 gene fragments detected by real- time PCR possibly correspond to viral DNA reminiscent from previous infections. The molecular characterization of viruses, based on the analysis of the complete or partial sequence of the vp2 gene, allowed typifying three viral strains of mongoose and four red fox's as feline panleukopenia virus (FPLV) and one stone marten's as newCPV- 2b type. The genetic similarity found between the FPLV viruses from free- ranging and captive wild species originated in Portugal and publicly available comparable sequences, suggests a closer genetic relatedness among FPLV circulating in Portugal. Although the clinical and epidemiological significance of infection could not be established, this study evidences that exposure of sympatric wild carnivores to parvovirus is common and geographically widespread, potentially carrying a risk to susceptible populations at the wildlife- domestic interface and to threatened species, such as the wildcat (Felis silvestris) and the critically endangered Iberian lynx (Lynx pardinus). (AU) | |
| FAPESP's process: | 11/00408-4 - Mesocarnivores adaptations to man shaped environments in two different spatio-temporal contexts: the eucaliptus production forests in Brazil and Portugal as case studies |
| Grantee: | Luis Miguel do Carmo Rosalino |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |