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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Doxorubicin induced dilated cardiomyopathy in a rabbit model: An update

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Author(s):
Gava, Fabio N. [1] ; Zacche, Evandro [1] ; Ortiz, Edna M. G. [1] ; Champion, Tatiana [1] ; Bandarra, Marcio B. [2] ; Vasconcelos, Rosemeri O. [2] ; Barbosa, Jose C. [3] ; Camacho, Aparecido A. [1]
Total Authors: 8
Affiliation:
[1] Sao Paulo State Univ, Div Vet Cardiol, Sao Paulo - Brazil
[2] Sao Paulo State Univ, Div Vet Pathol, Sao Paulo - Brazil
[3] Sao Paulo State Univ, Div Exact Sci, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Research in Veterinary Science; v. 94, n. 1, p. 115-121, FEB 2013.
Web of Science Citations: 20
Abstract

Dilated cardiomyopathy (DCM) is characterized by chamber dilation and cardiac dysfunction. Because of the poor prognosis, models are needed for the investigation of and development of new therapeutic approaches, as well as stem cell therapy. Doxorubicin (DOX), used as chemotherapeutic agent, is reported to be cumulative cardiotoxic causing DCM. The aim of the study was to investigate the onset of systolic dysfunction using echocardiography in rabbits receiving two different doses of DOX (1 mg/kg twice a week and 2 mg/kg once a week). Twenty rabbits were treated with doxorubicin in two different doses for 6 weeks and compared with a control group treated with NaCl 0.9%. The effect of doxorubicin on the myocardium was investigated with histological analysis and scanning electron microscopy of left ventricle (LV), as Well as in the interventricular septum (IVS) and right ventricle (RV). The results showed a high mortality rate for rabbits receiving 2 mg/kg once a week. A significant reduction in systolic function was present in animals treated with DOX after 6 weeks, with decreased ejection fraction and shortening fraction. Histology and electron microscopy revealed vacuolization, intracytoplasmic granulation, necrosis and interstitial fibrosis in LV, as well as in the IVS and RV. Doxorubicin induced changes are present in the LV, RV and IVS, and the administration at the dose of 1 mg/kg twice a week for only 6 weeks is safe and sufficient to induce DCM in rabbits. (C) 2012 Elsevier Ltd. All rights reserved. (AU)