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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Maternal protein restriction affects gene expression and enzyme activity of intestinal disaccharidases in adult rat offspring

Full text
Author(s):
D.F. Pinheiro [1] ; P.D.G. Pacheco [1] ; P.V. Alvarenga [1] ; J. Buratini Jr [1] ; A.C.S. Castilho [1] ; P.F. Lima [1] ; D.R.S. Sartori [1] ; M.L.M. Vicentini-Paulino [1]
Total Authors: 8
Affiliation:
[1] Univ Estadual Paulista, Inst Biociencias, Dept Fisiol, Botucatu, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Brazilian Journal of Medical and Biological Research; v. 46, n. 3, p. 287-292, 2013-03-15.
Abstract

This study investigated the consequences of intrauterine protein restriction on the gastrointestinal tract and particularly on the gene expression and activity of intestinal disaccharidases in the adult offspring. Wistar rat dams were fed isocaloric diets containing 6% protein (restricted, n = 8) or 17% protein (control, n = 8) throughout gestation. Male offspring (n = 5-8 in each group) were evaluated at 3 or 16 weeks of age. Maternal protein restriction during pregnancy produced offspring with growth restriction from birth (5.7 ± 0.1 vs 6.3 ± 0.1 g; mean ± SE) to weaning (42.4 ± 1.3 vs 49.1 ± 1.6 g), although at 16 weeks of age their body weight was similar to control (421.7 ± 8.9 and 428.5 ± 8.5 g). Maternal protein restriction also increased lactase activity in the proximal (0.23 ± 0.02vs 0.15 ± 0.02), medial (0.30 ± 0.06vs 0.14 ± 0.01) and distal (0.43 ± 0.07vs 0.07 ± 0.02 U·g-1·min-1) small intestine, and mRNA lactase abundance in the proximal intestine (7.96 ± 1.11vs 2.38 ± 0.47 relative units) of 3-week-old offspring rats. In addition, maternal protein restriction increased sucrase activity (1.20 ± 0.02 vs 0.91 ± 0.02 U·g-1·min-1) and sucrase mRNA abundance (4.48 ± 0.51 vs 1.95 ± 0.17 relative units) in the duodenum of 16-week-old rats. In conclusion, the present study shows for the first time that intrauterine protein restriction affects gene expression of intestinal enzymes in offspring. (AU)

FAPESP's process: 10/50616-0 - Expression of nutrients transporters and digestive enzymes in rats born to mothers submitted to protein restriction during pregnancy
Grantee:Maria de Lourdes Mendes Vicentini Paulino
Support Opportunities: Regular Research Grants