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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Prozone effects in microscopic agglutination tests for leptospirosis in the sera of mice infected with the pathogenic Leptospira interrogans serovar Canicola

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Author(s):
Fabio Hiroto Shimabukuro [1] ; Veruska Maia da Costa [2] ; Rodrigo Costa da Silva [3] ; Hélio Langoni [4] ; Aristeu Vieira da Silva [5] ; Lídia Raquel de Carvalho [6] ; Paulo Francisco Domingues [7]
Total Authors: 7
Affiliation:
[1] Instituto Adolfo Lutz. Centro de Laboratório Regional XI - Brasil
[2] Universidade Estadual Paulista. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Higiene Veterinária e Saúde Pública - Brasil
[3] Universidade Estadual Paulista. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Higiene Veterinária e Saúde Pública - Brasil
[4] Universidade Estadual Paulista. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Higiene Veterinária e Saúde Pública - Brasil
[5] Universidade Estadual de Feira de Santana. Departamento de Ciências Biológicas - Brasil
[6] Universidade Estadual Paulista. Instituto de Biociências. Departamento de Bioestatística - Brasil
[7] Universidade Estadual Paulista. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Higiene Veterinária e Saúde Pública - Brasil
Total Affiliations: 7
Document type: Journal article
Source: Memórias do Instituto Oswaldo Cruz; v. 108, n. 5, p. 668-670, 2013-08-00.
Abstract

Mice experimentally infected with a pathogenic strain of Leptospira interrogans serovar Canicola produced false negative results (prozone effect) in a microscopic agglutination test (MAT). This prozone effect occurred in several serum samples collected at different post-infection times, but it was more prominent in samples collected from seven-42 days post-infection and for 1:50 and 1:100 sample dilutions. This phenomenon was correlated with increased antibody titres in the early post-infection phase. While prozone effects are often observed in serological agglutination assays for the diagnosis of animal brucellosis and human syphilis, they are not widely reported in leptospirosis MATs. (AU)