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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Identification of Cryptosporidiumspecies and genotypes in dairy cattle in Brazil

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Author(s):
Flavio Medeiros Paz e Silva [1, 2] ; Raimundo Souza Lopes [2] ; João Pessoa Araújo-Junior [3]
Total Authors: 3
Affiliation:
[1] Univ Estadual Paulista UNESP, Inst Biociencias, Dept Microbiol Imunol, Lab Diagnost Mol, BR-18618970 Botucatu, SP - Brazil
[2] Univ Estadual Paulista UNESP, Fac Med Vet & Zootecnia, Dept Clin Vet, Lab Enfermidades Parasitarias, BR-18618970 Botucatu, SP - Brazil
[3] Universidade Estadual Paulista – UNESP. Instituto de Biociências. Departamento de Microbiologia-Imunologia - Brasil
Total Affiliations: 3
Document type: Journal article
Source: Revista Brasileira de Parasitologia Veterinária; v. 22, n. 1, p. 22-28, 2013-03-19.
Abstract

In this study, we identified Cryptosporidium species and genotypes present in dairy cattle in the central region of São Paulo state, Brazil. Fecal specimens were collected from 200 animals (100 calves and 100 cows) in ten dairy farms. Fecal samples were examined using microscopic examination (ME), enzyme immunoassay (EIA) and polymerase chain reaction (PCR). Cryptosporidiumspecies and genotypes were determined by restriction fragment length polymorphism (RFLP) or DNA sequencing analysis of the SSU-rRNA and GP60 genes. The occurrence of Cryptosporidium spp. infection was 14% (28/200). The occurrence in calves (26%) was significantly higher than in cows (2%). Of the 27 Cryptosporidium-positive specimens submitted to genotyping, C. andersoni was identified in 23 (85.1%), C. bovis in three (11.1%), and the zoonotic C. parvum subtype IIaA15G2R1 in one (3.7%). The study demonstrates thatCryptosporidium spp. infection was common and widespread in dairy cattle in this region and that calves have a high prevalence of C. andersoni. Furthermore, the presence of C. parvumsubtype IIaA15G2R1 indicates that dairy calves from this region should be considered a potential source of zoonotic Cryptosporidiumoocysts. (AU)