Cysticercosis: immunidiagnosis, antigens, immune response, clinical and epidemiolo...
| Full text | |
| Author(s): |
Silvana de Cássia Paulan
[1]
;
Rutilia Marisela Hernándes Gonzáles
[2]
;
Laura Adalid Peralta
[3]
;
Josy Campanhã Vicentini-Oliveira
[4]
;
Germano Francisco Biondi
[5]
;
Edda Sciuto Conde
[3]
;
Robert Michael Evans Parkhouse
[6]
;
Cáris Maroni Nunes
[8]
Total Authors: 8
|
| Affiliation: | [1] Universidade Estadual Paulista “Júlio de Mesquita Filho” – UNESP. Produção e Saúde Animal. Departamento de Apoio - Brasil
[2] Universidad Nacional Autónoma de México. Instituto de Investigaciones Biomédicas - México
[3] Univ Nacl Autonoma Mexico, Inst Invest Biomed, Mexico City 04510, DF - Mexico
[4] Universidade Estadual Paulista “Júlio de Mesquita Filho” – UNESP. Escola de Medicina Veterinária e Zootecnia - Brasil
[5] Univ Estadual Paulista Julio de Mesquita Filho UN, Escola Med Vet & Zootecnia, BR-16050680 Aracatuba, SP - Brazil
[6] Inst Gulbenkian Ciencias, Oeiras - Portugal
[7] Universidade Estadual Paulista “Júlio de Mesquita Filho” – UNESP. Produção e Saúde Animal. Departamento de Apoio - Brasil
Total Affiliations: 7
|
| Document type: | Journal article |
| Source: | Revista Brasileira de Parasitologia Veterinária; v. 22, n. 2, p. 270-275, 2013-06-25. |
| Abstract | |
Bovine cysticercosis, a cosmopolitan disease caused by Taenia saginata, leads to economic losses due to carcass devaluation at slaughter. Sanitary inspection at slaughterhouses, the routine diagnostic method in Brazil, lacks the necessary sensitivity to detect the mildly infected cattle that are typically encoutered in Brazil. In this study we have tested cattle sera from animals diagnosed as positive and negative by veterianry inspection for (1) anti-parasite antibodies using metacestodes antigens (T. solium vesicular fluid and T. saginata secretions) and (2) the HP10 secreted antigen of viable metacestodes. The cut-off values were calculated by ROC curve for intense and mild infections conditions, and by the classical method ( for negative samples). The sensitivity and specificity of these diagnostic tests were different depending on the assumed cut-off value and, importantly, whether the infection was mild or intense. In spite of these observations, however, such ELISA assays for serum antibodies and parasite antigens constitute an important tool for epidemiological porposes, and in establishing priorities for the control of bovine cysticercosis. (AU) | |
| FAPESP's process: | 09/12719-4 - DEVELOPMENT OF SANDWICH ELISA FOR BOVINE CYSTICERCOSIS |
| Grantee: | Silvana de Cássia Paulan |
| Support Opportunities: | Scholarships in Brazil - Master |