| Full text | |
| Author(s): |
Filippo, Thais R. M.
[1]
;
Galindo, Layla T.
[2, 1]
;
Barnabe, Gabriela F.
[3]
;
Ariza, Carolina B.
[1]
;
Mello, Luiz E.
[2, 3]
;
Juliano, Maria A.
[4]
;
Juliano, Luiz
[4]
;
Porcionatto, Marimelia A.
[2, 1]
Total Authors: 8
|
| Affiliation: | [1] Univ Fed Sao Paulo, Escola Paulista Med, Dept Biochem, Sao Paulo - Brazil
[2] Univ Fed Sao Paulo, Neurobiol Lab, Sao Paulo - Brazil
[3] Univ Fed Sao Paulo, Escola Paulista Med, Dept Physiol, Sao Paulo - Brazil
[4] Univ Fed Sao Paulo, Escola Paulista Med, Dept Biophys, Sao Paulo - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | STEM CELL RESEARCH; v. 11, n. 2, p. 913-925, SEP 2013. |
| Web of Science Citations: | 24 |
| Abstract | |
Neural stem/progenitor cells (NSC) respond to injury after brain injuries secreting IL-1, IL-6, TNF-alpha, IL-4 and IL-10, as well as chemokine members of the CC and CXC ligand families. CXCL12 is one of the chemokines secreted at an injury site and is known to attract NSC-derived neuroblasts, cells that express CXCL12 receptor, CXCR4. Activation of CXCR4 by CXCL12 depends on two domains located at the N-terminal of the chemokine. In the present work we aimed to investigate if the N-terminal end of CXCL12, where CXCR4 binding and activation domains are located, was sufficient to induce NSC-derived neuroblast chemotaxis. Our data show that a synthetic peptide analogous to the first 21 amino acids of the N-terminal end of CXCL12, named PepC-C (KPVSLSYRCPCRFFESHIARA), is able to promote chemotaxis of neuroblasts in vivo, and stimulate chemotaxis and proliferation of CXCR4+ cells in vitro, without affecting NSC fate. We also show that PepC-C upregulates CXCL12 expression in vivo and in vitro. We suggest the N-terminal end of CXCL12 is responsible for a positive feedback loop to maintain a gradient of CXCL12 that attracts neuroblasts from the subventricular zone into an injury site. (C) 2013 The Authors. Published by Elsevier B.V. All rights reserved. (AU) | |
| FAPESP's process: | 12/00652-5 - Molecular mechanisms of neural stem cells migration, survival and differentiation |
| Grantee: | Marimélia Aparecida Porcionatto |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 05/04061-8 - Cell therapy in the regeneration of central nervous system injuries: transplantation of bone marrow stem cells expressing chondroitinase AC |
| Grantee: | Marimélia Aparecida Porcionatto |
| Support Opportunities: | Regular Research Grants |