AMPK signaling pathway is rapidly activated by T3 and regulates the cardiomyocyte growth

Author(s)

Cremasco Takano, Ana Paula ^[1] ; Diniz, Gabriela Placona ^[1] ; Morais Barreto-Chaves, Maria Luiza ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Anat, Lab Cellular Biol & Funct Anat, BR-05508900 Sao Paulo - Brazil

• Document type: Journal article
• Source: Molecular and Cellular Endocrinology; v. 376, n. 1-2, p. 43-50, AUG 25 2013.
• Web of Science Citations: 8

Abstract

Previous studies have indicated that AMP-activated protein kinase (AMPK) plays a critical role in the control of cardiac hypertrophy mediated by different stimuli such as thyroid hormone (TH). Although the classical effects of TH mediating cardiac hypertrophy occur by transcriptional mechanisms, recent studies have identified other responses to TH, which are more rapid and take place in seconds or minutes evidencing that TH rapidly modulates distinct signaling pathway, which might contribute to the regulation of cardiomyocyte growth. Here, we evaluated the rapid effects of TH on AMPK signaling pathway in cultured cardiomyocytes and determined the involvement of AMPK in T3-induced cardiomyocyte growth. We found for the first time that T3 rapidly activated AMPK signaling pathway. The use of small interfering RNA against AMPK resulted in increased cardiomyocyte hypertrophy while the pharmacological stimulation of AMPK attenuated this process, demonstrating that AMPK contributes to regulation of T3-induced cardiomyocyte growth. (C) 2013 Elsevier Ireland Ltd. All rights reserved. (AU)

• FAPESP's process: 09/16888-5 - Role of AMPK in the cardiac hypertrophy induced by thyroid hormone. Contribution of angiotensin II receptors.
• Grantee: Maria Luiza de Morais Barreto de Chaves
• Support Opportunities: Regular Research Grants
• FAPESP's process: 09/09922-2 - Role of AMPK in the cardiac hypertrophy induced by Thyroid Hormone.
• Grantee: Ana Paula Cremasco Takano
• Support Opportunities: Scholarships in Brazil - Master