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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Peripheral kappa and delta opioid receptors are involved in the antinociceptive effect of crotalphine in a rat model of cancer pain

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Brigatte, Patricia [1, 2] ; Konno, Katsuhiro [3] ; Gutierrez, Vanessa Pacciari [2, 1] ; Sampaio, Sandra Coccuzzo [2, 1] ; Zambelli, Vanessa Olzon [2, 1] ; Picolo, Gisele [2, 1] ; Curi, Rui [4] ; Cury, Yara [2, 1]
Total Authors: 8
[1] Inst Butantan, Lab Especial Dor & Sinalizacao, BR-05503900 Sao Paulo - Brazil
[2] Inst Butantan, Lab Fisiopatol, BR-05503900 Sao Paulo - Brazil
[3] Inst Butantan, Ctr Toxinol Aplicada, BR-05503900 Sao Paulo - Brazil
[4] Univ Sao Paulo, Inst Ciencias Biomed, Dept Fisiol & Biofis, BR-05508900 Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Pharmacology Biochemistry and Behavior; v. 109, p. 1-7, AUG 2013.
Web of Science Citations: 8

Cancer pain is an important clinical problem and may not respond satisfactorily to the current analgesic therapy. We have characterized a novel and potent analgesic peptide, crotalphine, from the venom of the South American rattlesnake Crotalus durissus terrificus. In the present work, the antinociceptive effect of crotalphine was evaluated in a rat model of cancer pain induced by intraplantar injection of Walker 256 carcinoma cells. Intraplantar injection of tumor cells caused the development of hyperalgesia and allodynia, detected on day 5 after tumor cell inoculation. Crotalphine (6 mu g/kg), administered p.o., blocked both phenomena. The antinociceptive effect was detected 1 h after treatment and lasted for up to 48 h. Intraplantar injection of nor-binaltorphimine (50 g/paw), a selective antagonist of kappa-opioid receptors, antagonized the antinociceptive effect of the peptide, whereas N,N-diallyl-Tyr-Aib-Phe-Leu (ICI 174,864, 10 mu g/paw), a selective antagonist of delta-opioid receptors, partially reversed this effect. On the other hand, D-Phe-Cys-Tyr-D-Trp-Orn-Thr-Pen-Thr amide (CTOP, 20 g/paw), an antagonist of mu-opioid receptors, did not modify crotalphine-induced antinociception. These data indicate that crotalphine induces a potent and long lasting opioid-mediated antinociception in cancer pain. (C) 2013 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 08/57898-0 - National Institute of Science and Technology on Toxins
Grantee:Osvaldo Augusto Brazil Esteves Sant'Anna
Support type: Research Projects - Thematic Grants
FAPESP's process: 98/14307-9 - Center for Applied Toxinology
Grantee:Hugo Aguirre Armelin
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC