| Full text | |
| Author(s): |
Total Authors: 4
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| Affiliation: | [1] Sociedade Beneficente Israelita Brasileira Albert Einstein (SBIBAE). Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE) - Brasil
[4] Universidade de São Paulo (USP). Faculdade de Medicina. Departamento de Neurologia - Brasil
Total Affiliations: 4
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| Document type: | Journal article |
| Source: | Biochimica et Biophysica Acta (BBA) : Gene Structure and Expression; v. 1769, n. 7/8, p. 437-442, July 2007. |
| Field of knowledge: | Health Sciences - Medicine |
| Abstract | |
Comparative analysis of cancer stem cells with their neoplastic and non-neoplastic counterparts should help better understand the underlying molecular events leading to transformation and tumor dissemination. Here, we report a molecular signature comprised by genes with exclusive aberrant expression in CD133+ cells, a reported subpopulation of tumorigenic stem-like cells, isolated from human glioblastomas. Microarrays covering 55,000 transcripts were used to compare gene expression profiles in purified subpopulations of CD133+ and CD133- GBM cells. Sixteen genes, many of which not previously associated with astrocytomas, were found aberrantly expressed in CD133+ cells, but not in CD133-, when compared with corresponding non-neoplastic controls. Up-regulation of two of such genes, E2F2 and HOXC9, was detected in a set of 54 astrocytomas of different grades and significantly associated with malignancy. Due to their distinctive expression in CD133+ cells, the use of E2F2 and HOXC9 as therapeutic targets for tumor eradication is suggested. (AU) | |