| Full text | |
| Author(s): |
Dariolli, Rafael
[1]
;
Bassaneze, Vinicius
[1]
;
Nakamuta, Juliana Sanajotti
[1]
;
Omae, Samantha Vieira
[1]
;
Cristina, Luciene
[1]
;
Campos, Gastalho
[1]
;
Krieger, Jose E.
[1]
Total Authors: 7
|
| Affiliation: | [1] Univ Sao Paulo, Sch Med, Heart Inst InCor, Sao Paulo - Brazil
Total Affiliations: 1
|
| Document type: | Journal article |
| Source: | PLoS One; v. 8, n. 7 JUL 9 2013. |
| Web of Science Citations: | 19 |
| Abstract | |
We and others have provided evidence that adipose tissue-derived mesenchymal stem cells (ASCs) can mitigate rat cardiac functional deterioration after myocardial ischemia, even though the mechanism of action or the relevance of these findings to human conditions remains elusive. In this regard, the porcine model is a key translational step, because it displays heart anatomic-physiological features that are similar to those found in the human heart. Towards this end, we wanted to establish the cultural characteristics of porcine ASCs (pASCs) with or without long-term cryostorage, considering that allogeneic transplantation may also be a future option. Compared to fresh pASCs, thawed cells displayed 90-95% viability and no changes in morphological characteristics or in the expression of surface markers (being pASCs characterized by positive markers CD29(+); CD90(+); CD44(+); CD140b(+); CD105(+); and negative markers CD31(-); CD34(-); CD45(-) and SLA-DR-; n = 3). Mean population doubling time was also comparable (64.26 +/- 15.11 hours to thawed cells vs. 62.74 +/- 18.07 hours to fresh cells) and cumulative population doubling increased constantly until Passage 10 (P10) in the entire cell population, with a small and gradual increase in senescence (P5, 3.25% +/- 0.26 vs. 3.47%+/- 0.32 and P10, 9.6%+/- 0.29 vs. 10.67%+/- 1.25, thawed vs. fresh; SA-beta-Gal staining). Chromosomal aberrations were not observed. In addition, under both conditions pASCs responded to adipogenic and osteogenic chemical cues in vitro. In conclusion, we have demonstrated the growth characteristics, senescence, and the capacity of pASCs to respond to chemical cues in vitro and have provided evidence that these properties are not influenced by cryostorage in 10% DMSO solution. (AU) | |
| FAPESP's process: | 08/52436-9 - Reprogramação dirigida de células mesenquimais de tecido adiposo em células-tronco pluripotentes e cardiomiócitos |
| Grantee: | José Eduardo Krieger |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 08/52334-1 - Reprogramacao de celulas mesenquimais de tecido adiposo humano em celulas-tronco pluripotentes por meio de proteina de fusao tat. |
| Grantee: | Vinícius Bassaneze |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 09/50624-5 - Avaliacao dos mecanismos regulatorios da crp3/mlp durante a arterializacao de enxertos venosos em sistemas ex vivo, in vitro e in vivo. |
| Grantee: | Luciene Cristina Gastalho Campos |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 08/52335-8 - Reprogramacao de fibroblastos em celulas-tronco pluripotentes e diferenciacao destas em cardiomiocitos por meio da expressao de fatores de transcricao cardiacos. |
| Grantee: | Chester Bittencourt Sacramento |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 08/55918-4 - Identificacao de alvos proteicos com potencial diagnosticos em doencas arterial coronariana. |
| Grantee: | Gabriela Venturini da Silva |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 10/52516-2 - Secretoma de celulas-tronco do tecido adiposo submetidas ao estiramento sob isquemia in vitro: manipulacao do microambiente para |
| Grantee: | Juliana Sanajotti Nakamuta |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 07/58942-0 - From the bench to clinical trials: development of biomarkers as response predictors to therapy and target organs damage in systemic arterial hypertension |
| Grantee: | Eduardo Moacyr Krieger |
| Support Opportunities: | Research Projects - Thematic Grants |