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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Myocardial infarction area quantification using high-resolution SPECT images in rats

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Lemos de Oliveira, Luciano Fonseca [1] ; Mejia, Jorge [2] ; Vieira de Carvalho, Eduardo Elias [1] ; Lataro, Renata Maria [1] ; Frassetto, Sarita Nasbine [1] ; Fazan, Jr., Rubens [3] ; Salgado, Helio Cesar [3] ; Galvis-Alonso, Orfa Yineth [2] ; Simoes, Marcus Vinicius [1]
Total Authors: 9
[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Clin Med, Div Cardiol, BR-14049 Ribeirao Preto, SP - Brazil
[2] Fac Med Sao Jose do Rio Preto, Sao Jose Do Rio Preto, SP - Brazil
[3] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Fisiol, BR-14049 Ribeirao Preto, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Arquivos Brasileiros de Cardiologia; v. 101, n. 1, p. 59-67, JUL 2013.
Web of Science Citations: 7

BACKGROUND: Imaging techniques enable in vivo sequential assessment of the morphology and function of animal organs in experimental models. We developed a device for high-resolution single photon emission computed tomography (SPECT) imaging based on an adapted pinhole collimator. OBJECTIVE: To determine the accuracy of this system for quantification of myocardial infarct area in rats. METHODS: Thirteen male Wistar rats (250 g) underwent experimental myocardial infarction by occlusion of the left coronary artery. After 4 weeks, SPECT images were acquired 1.5 hours after intravenous injection of 555 MBq o f 99mTc-Sestamibi. The tomographic reconstruction was performed by using specially developed software based on the Maximum Likelihood algorithm. The analysis of the data included the correlation between the area of perfusion defects detected by scintigraphy and extent of myocardial fibrosis assessed by histology. RESULTS: The images showed a high target organ/background ratio with adequate visualization of the left ventricular walls and cavity. All animals presenting infarction areas were correctly identified by the perfusion images. There was no difference of the infarct area as measured by SPECT (21.1 ± 21.2%) and by histology (21.7 ± 22.0%; p=0.45). There was a strong correlation between individual values of the area of infarction measured by these two methods. CONCLUSION: The developed system presented adequate spatial resolution and high accuracy for the detection and quantification of myocardial infarction areas, consisting in a low cost and versatile option for high-resolution SPECT imaging of small rodents. (AU)

FAPESP's process: 11/03261-4 - Study of the myocardial sympathetic innervation disturbance and myocardial perfusion changes and their correlation with the progression of the left ventricular dysfunction in an experimental model of chronic Changes cardiomyopathy
Grantee:Marcus Vinicius Simões
Support Opportunities: Regular Research Grants