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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effects of chronic passive smoking on the regeneration of rat femoral defects filled with hydroxyapatite and stimulated by laser therapy

Full text
Author(s):
Franco, G. R. [1] ; Laraia, I. O. [1] ; Maciel, A. A. W. [1] ; Miguel, N. M. [1] ; dos Santos, G. R. [1] ; Fabrega-Carvalho, C. A. [1] ; Pinto, C. A. L. [1] ; Pettian, M. S. [1] ; Cunha, M. R. [1]
Total Authors: 9
Affiliation:
[1] Fac Med Jundiai, Dept Morphol & Pathol, BR-13202550 Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: INJURY-INTERNATIONAL JOURNAL OF THE CARE OF THE INJURED; v. 44, n. 7, p. 908-913, JUL 2013.
Web of Science Citations: 11
Abstract

Defects associated with bone mass loss are frequently treated by autogenous bone grafting. However, synthetic biomaterials such as calcium phosphate ceramics can substitute autologous grafts as long as they are biocompatible with bone tissue. In addition, low-level laser therapy (LLLT) is used to enhance bone regeneration by stimulating the local microcirculation and increasing the synthesis of collagen by bone cells. However, bone health is fundamental for osseointegration of the graft and bone repair. In this respect, excessive tobacco consumption can compromise expected outcomes because of its deleterious effects on bone metabolism that predispose to the development of osteoporosis. The objective of this study was to evaluate the regeneration of bone defects implanted with biomaterial and stimulated by LLLT in rats submitted to passive cigarette smoking. Porous hydroxyapatite granules were implanted into critical-size defects induced experimentally in the distal epiphysis of the right femur of 20 female Wistar rats submitted to passive smoking for 8 months in a smoking box. The defect site was irradiated with a gallium-arsenide laser at an intensity of 5.0 J/cm(2). The animals were divided into four groups: control (non-smoking) rates submitted (G2) or not (G1) to laser irradiation, and smoking rats submitted (G4) or not (G3) to laser irradiation. The animals were sacrificed 8 weeks after biomaterial implantation. The right femurs were removed for photodocumentation, radiographed, and processed for routine histology. The results showed good radiopacity of the implant site and of the hydroxyapatite granules. Histologically, formation of new trabecular bone was observed adjacent to the hydroxyapatite granules in G1 and G2. In G3 and G4, the granules were surrounded mainly by connective tissue. In conclusion, passive smoking compromised bone neoformation in the defects and the LLLT protocol was not adequate to stimulate local osteogenesis. (C) 2013 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 10/05769-2 - EFFECTS OF THE PASSIVE TOBACCO SMOKING IN THE BONE DEFECTS REGENERATION FILLED WITH HYDROXYAPATITE ASSOCIATED TO THE COLLAGEN AND STIMULATED BY THE LASERTERAPIA IN RATS
Grantee:Guacira Rovigatti Franco
Support Opportunities: Scholarships in Brazil - Scientific Initiation