Interval Multivariate Curve Resolution in the Dereplication of HPLC-DAD Data from Jatropha gossypifolia

Author(s)

Pilon, Alan Cesar ^[1] ; Carneiro, R. L. ^[2] ; Carnevale Neto, F. ^[1] ; Bolzani, Vanderlan da S. ^[1] ; Castro-Gamboa, I. ^[1]

Affiliation

^[1] Sao Paulo State Univ, Inst Chem, Dept Organ Chem, Nucleus Bioassays Biosynth & Ecophysiol Nat Prod, BR-14800900 Sao Paulo - Brazil

^[2] Univ Fed Sao Carlos, CCET, Dept Chem, BR-13565905 Sao Paulo - Brazil

• Document type: Journal article
• Source: Phytochemical Analysis; v. 24, n. 4, p. 401-406, JUL-AUG 2013.
• Web of Science Citations: 16

Abstract

Introduction Jatropha gossypifolia has been used quite extensively by traditional medicine for the treatment of several diseases in South America and Africa. This medicinal plant has therapeutic potential as a phytomedicine and therefore the establishment of innovative analytical methods to characterise their active components is crucial to the future development of a quality product. Objective To enhance the chromatographic resolution of HPLC-UV-diode-array detector (DAD) experiments applying chemometric tools. Methods Crude leave extracts from J. gossypifolia were analysed by HPLC-DAD. A chromatographic band deconvolution method was designed and applied using interval multivariate curve resolution by alternating least squares (MCR-ALS). Results The MCR-ALS method allowed the deconvolution from up to 117% more bands, compared with the original HPLC-DAD experiments, even in regions where the UV spectra showed high similarity. The method assisted in the dereplication of three C-glycosylflavones isomers: vitexin/isovitexin, orientin/homorientin and schaftoside/isoschaftoside. Conclusion The MCR-ALS method is shown to be a powerful tool to solve problems of chromatographic band overlapping from complex mixtures such as natural crude samples. Copyright (c) 2013 John Wiley \& Sons, Ltd. (AU)

• FAPESP's process: 03/02176-7 - Conservation and sustainable use of the diversity from Cerrado and Atlantic Forest: chemical diversity and prospecting for potential drugs - phase II
• Grantee: Vanderlan da Silva Bolzani
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
• FAPESP's process: 10/16520-5 - Application of calibration chemometrics methods and multivariate curve resolution in Raman spectroscopy for qualitative, quantitative and polymorphism analyses in medicines
• Grantee: Renato Lajarim Carneiro
• Support Opportunities: Regular Research Grants