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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Interval Multivariate Curve Resolution in the Dereplication of HPLC-DAD Data from Jatropha gossypifolia

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Author(s):
Pilon, Alan Cesar [1] ; Carneiro, R. L. [2] ; Carnevale Neto, F. [1] ; Bolzani, Vanderlan da S. [1] ; Castro-Gamboa, I. [1]
Total Authors: 5
Affiliation:
[1] Sao Paulo State Univ, Inst Chem, Dept Organ Chem, Nucleus Bioassays Biosynth & Ecophysiol Nat Prod, BR-14800900 Sao Paulo - Brazil
[2] Univ Fed Sao Carlos, CCET, Dept Chem, BR-13565905 Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Phytochemical Analysis; v. 24, n. 4, p. 401-406, JUL-AUG 2013.
Web of Science Citations: 16
Abstract

Introduction Jatropha gossypifolia has been used quite extensively by traditional medicine for the treatment of several diseases in South America and Africa. This medicinal plant has therapeutic potential as a phytomedicine and therefore the establishment of innovative analytical methods to characterise their active components is crucial to the future development of a quality product. Objective To enhance the chromatographic resolution of HPLC-UV-diode-array detector (DAD) experiments applying chemometric tools. Methods Crude leave extracts from J. gossypifolia were analysed by HPLC-DAD. A chromatographic band deconvolution method was designed and applied using interval multivariate curve resolution by alternating least squares (MCR-ALS). Results The MCR-ALS method allowed the deconvolution from up to 117% more bands, compared with the original HPLC-DAD experiments, even in regions where the UV spectra showed high similarity. The method assisted in the dereplication of three C-glycosylflavones isomers: vitexin/isovitexin, orientin/homorientin and schaftoside/isoschaftoside. Conclusion The MCR-ALS method is shown to be a powerful tool to solve problems of chromatographic band overlapping from complex mixtures such as natural crude samples. Copyright (c) 2013 John Wiley \& Sons, Ltd. (AU)

FAPESP's process: 03/02176-7 - Conservation and sustainable use of the diversity from Cerrado and Atlantic Forest: chemical diversity and prospecting for potential drugs - phase II
Grantee:Vanderlan da Silva Bolzani
Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
FAPESP's process: 10/16520-5 - Application of calibration chemometrics methods and multivariate curve resolution in Raman spectroscopy for qualitative, quantitative and polymorphism analyses in medicines
Grantee:Renato Lajarim Carneiro
Support Opportunities: Regular Research Grants