| Full text | |
| Author(s): |
de Moura, Claudia
[1]
;
Tiba, Monique Ribeiro
[2]
;
da Silva, Marcio Jose
[3]
;
Leite, Domingos da Silva
[2]
Total Authors: 4
|
| Affiliation: | [1] Univ Paulista UNIP, Inst Ciencias Saude, BR-13214525 Jundiai, SP - Brazil
[2] Univ Estadual Campinas Unicamp, Inst Biol, Dept Genet Evolucao & Bioagentes, Lab Antigenos Bacterianos 2, BR-13083970 Campinas, SP - Brazil
[3] Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, Inst Biol, Campinas - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Pesquisa Veterinária Brasileira; v. 33, n. 4, p. 417-422, APR 2013. |
| Web of Science Citations: | 1 |
| Abstract | |
Identification of Escherichia coli requires knowledge regarding the prevalent serotypes and virulence factors profiles allows the classification in pathogenic/non-pathogenic. However, some of these bacteria do not express flagellar antigen invitro. In this case the PCR-restriction fragment length polymorphism (RFLP-PCR) and sequencing of the fliC may be suitable for the identification of antigens by replacing the traditional serology. We studied 17 samples of E. coli isolated from animals and presenting antigen H nontypeable (HNT). The H antigens were characterized by PCR-RFLP and sequencing of fliC gene. Three new flagellin genes were identified, for which specific antisera were obtained. The PCR-RFLP was shown to be faster than the serotyping H antigen in E. coli, provided information on some characteristics of these antigens and indicated the presence of new genes fliC. (AU) | |