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| Author(s): |
Icaro Matioli Barbosa
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2011-12-15 |
| Examining board members: |
Silvia Beatriz Boscardin;
Karen Spadari Ferreira;
Claudio Romero Farias Marinho
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| Advisor: | Silvia Beatriz Boscardin |
| Abstract | |
Dendritic cells (DCs) are cells of the immune system very important in the process of induction of immunity. They are able to connect innate and acquired immune responses and lead to activation of T and B cells. Recently it was shown that it is possible to target antigens directly to DCs in vivo by the administration of low doses of a recombinant hybrid protein consisting of a monoclonal antibody specific for receptors present on the surface of these cells fused with the antigen of interest. When these hybrid antibodies were injected in animals in the presence of a DC maturation stimulus, strong immune response against different antigens was obtained. Two of the monoclonal antibodies used have the ability to bind to either the DEC205 or the DCIR2 endocytic receptors present on the surface of two distinct DC sub-populations. In this work, we constructed hybrid antibodies fused with the sequence encoding the MSP-119 protein present on the surface P. yoelii and P. vivax merozoites. Most antibodies were successfully produced and maintained their ability to bind to their respective receptors. Immunization trials showed that the anti-DEC antibody fused to any of the two proteins was able to induce mainly a cellular immune response when administered in the presence of adjuvants. On the other hand, the humoral immune response depends of some combinations. (AU) | |
| FAPESP's process: | 09/12625-0 - Targeting the Plasmodium vivax and Plasmodium yoelii MSP-119 proteins to dendritic cells in vivo: analysis of cellular and humoral immune responses. |
| Grantee: | Icaro Matioli Barbosa |
| Support Opportunities: | Scholarships in Brazil - Master |