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Investigation of hyperfine interactions in DNA and antibody of different lineages of mice infected by T. cruzi by perturbed gamma-gamma angular correlation spectroscopy

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Author(s):
Andréia dos Santos Silva
Total Authors: 1
Document type: Doctoral Thesis
Press: São Paulo.
Institution: Universidade de São Paulo (USP). Instituto de Pesquisas Energéticas e Nucleares (IPEN/BT)
Defense date:
Examining board members:
Artur Wilson Carbonari; Ana Maria da Costa Ferreira; Eduardo Landulfo; André Luis Lapolli; Silvia Maria de Paula
Advisor: Artur Wilson Carbonari
Abstract

In the present work perturbed angular correlation (PAC) spectroscopy was used to measured electric quadrupole interactions in DNA biomolecules of different mice lineages (A/J, C57BL/6, B6AF1, BXA1 e BXA2), samples of different isotypes of immunoglobulin G (IgG1, IgG2a e IgG2b) and active portions of complete and fragmented immunoglobulin responsible by the immune response. Electric quadrupole interactions were also measured in DNA nitrogenous bases (adenine, cytosine, guanine, thymine). PAC measurements were performed using 111In111Cd; 111mCd111Cd; 111Ag111Cd; e 181Hf181Ta as probe nuclei, and carried out at room temperature and liquid nitrogen temperature, in order to investigate dynamic and static hyperfine interactions, respectively. The biomolecule samples were directly marked with the radioactive parent nuclei, whose atom link to a certain site in the biomolecules. The biological materials as well as the probe nuclei were chosen to investigate the possibility to use PAC spectroscopy to measure hyperfine parameters at nuclei from metallic elements bound to biomolecules (including the use of different probe nuclei produced in the decay of parent nuclei of four different metals) and also to study the behavior of different biomolecules by means of the measured hyperfine parameters. Results show differences in the hyperfine interactions of probe nuclei bound to the studied biomolecules. Such differences were observed by variations in the hyperfine parameters, which depend on the type of biomolecule and the results also show that the probe nuclei atom bound to the molecule in some cases and in others do not. (AU)