Assessing stir bar sorptive extraction and microextraction by packed sorbent for determination of selective serotonin reuptake inhibitor antidepressants in plasma sample by non-aqueous capillary electrophoresis

Author(s)

Ana Paula F. Catai ^[1] ; Flávio P. Picheli ^[2] ; Emanuel Carrilho ^[3] ; Maria Eugênia C. Queiroz ^[4]

Affiliation

^[1] Univ Sao Paulo, Dept Quim, Fac Filosofia Ciencias & Letras Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Universidade de São Paulo. Instituto de Química de São Carlos - Brasil

^[3] Univ Sao Paulo, Inst Quim Sao Carlos, BR-13560970 Sao Carlos, SP - Brazil

^[4] Universidade de São Paulo. Faculdade de Filosofia Ciências e Letras de Ribeirão Preto. Departamento de Química - Brasil

• Document type: Journal article
• Source: Journal of the Brazilian Chemical Society; v. 24, n. 10, p. 1635-1641, 2013-10-00.

Abstract

Two approaches based on partition equilibrium, stir bar sorptive extraction (SBSE) and microextraction by packed sorbent (MEPS) were evaluated for determination of selective serotonin reuptake inhibitor antidepressants (SSRI): fluoxetine, sertraline, citalopram and paroxetine in plasma sample by non-aqueous capillary electrophoresis (NACE) with spectrophotometric detection. The SBSE/NACE and MEPS/NACE methods showed linear range from the LOQ up to 500 ng mL-1. LOQ values ranged from 10 to 30 ng mL(-1)1 in both techniques, depending on the compound. The inter-assay precision of the methods presented coefficient of variation lower than 12%. According to the analytical validation results, the methods are adequate for SSRI antidepressant analyses at therapeutic levels, consequently, amenable to therapeutic drug monitoring. These methods reduced the volumes of biological samples, the use of organic solvents and the number of steps involved in sample preparation. (AU)