Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Isolation and characterization of equine peripheral blood-derived multipotent mesenchymal stromal cells

Full text
Author(s):
Armando de M. Carvalho [1] ; Ana Lucia M. Yamada [1] ; Juliana R.B. Martins [2] ; Leandro Maia [3] ; Marjorie A. Golim [2] ; Elenice Deffune [2] ; Carlos A. Hussni [1] ; Ana Liz G. Alves [1]
Total Authors: 8
Affiliation:
[1] Univ Estadual Paulista Unesp, Dept Cirurgia & Anestesiol Vet, Fac Med Vet & Zootecnia FMVZ, BR-18618970 Botucatu, SP - Brazil
[2] UNESP, FMB, Hemoctr, Botucatu, SP - Brazil
[3] FMVZ Unesp, Dept Clin Vet, Botucatu, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Pesquisa Veterinária Brasileira; v. 33, n. 9, p. 1151-1154, 2013-09-00.
Abstract

The objective of the study was to isolate, cultivate and characterize equine peripheral blood-derived multipotent mesenchymal stromal cells (PbMSCs). Peripheral blood was collected, followed by the isolation of mononuclear cells using density gradient reagents, and the cultivation of adherent cells. Monoclonal mouse anti-horse CD13, mouse anti-horse CD44, and mouse anti-rat CD90 antibodies were used for the immunophenotypic characterization of the surface of the PbMSCs. These cells were also cultured in specific media for adipogenic and chondrogenic differentiation. There was no expression of the CD13 marker, but CD44 and CD90 were expressed in all of the passages tested. After 14 days of cell differentiation into adipocytes, lipid droplets were observed upon Oil Red O (ORO) staining. Twenty-one days after chondrogenic differentiation, the cells were stained with Alcian Blue. Although the technique for the isolation of these cells requires improvement, the present study demonstrates the partial characterization of PbMSCs, classifying them as a promising type of progenitor cells for use in equine cell therapy. (AU)

FAPESP's process: 09/10670-8 - Autologous implant of mesenchymal stem cells and platelet-rich plasma associated in treatment of induced tendinitis in horses
Grantee:Armando de Mattos Carvalho
Support Opportunities: Scholarships in Brazil - Doctorate