Interactive effects of mechanical ventilation, inhaled nitric oxide and oxidative stress in acute lung injury

Author(s)

Ronchi, Carlos Fernando ^{[1, 2, 3]} ; Anjos Ferreira, Ana Lucia ^[4] ; Campos, Fabio Joly ^[1] ; Kurokawa, Cilmery Suemi ^[1] ; Carpi, Mario Ferreira ^[1] ; Moraes, Marcos Aurelio ^[1] ; Bonatto, Rossano Cesar ^[1] ; Yeum, Kyung-Jin ^[2] ; Fioretto, Jose Roberto ^[1]

Affiliation

^[1] Sao Paulo State Univ, UNESP, Botucatu Med Sch, Dept Pediat, Botucatu, SP - Brazil

^[2] Tufts Univ, Jean Mayer USDA, Human Nutr Res Ctr Aging, Boston, MA 02111 - USA

^[3] Univ Sagrado Coracao, Dept Hlth Sci, BR-17011160 Bauru, SP - Brazil

^[4] Sao Paulo State Univ, UNESP, Botucatu Med Sch, Dept Internal Med, Botucatu, SP - Brazil

• Document type: Journal article
• Source: Respiratory Physiology & Neurobiology; v. 190, n. 1, p. 118-123, JAN 2014.
• Web of Science Citations: 6

Abstract

To compare conventional mechanical ventilation (CMV) and high-frequency oscillatory ventilation (HFOV), with/without inhaled nitric oxide (iNO), for oxygenation, inflammation, antioxidant/oxidative stress status, and DNA damage in a model of acute lung injury (ALI). Lung injury was induced by tracheal infusion of warm saline. Rabbits were ventilated at F-IO2 1.0 and randomly assigned to one of five groups. Overall antioxidant defense/oxidative stress was assessed by total antioxidant performance assay, and DNA damage by comet assay. Ventilatory and hemodynamic parameters were recorded every 30 min for 4 h. ALI groups showed worse oxygenation than controls after lung injury. After 4 h of mechanical ventilation, HFOV groups presented significant improvements in oxygenation. HFOV with and without iNO, and CMV with iNO showed significantly increased antioxidant defense and reduced DNA damage than CMV without iNO. Inhaled nitric oxide did not beneficially affect HFOV in relation to antioxidant defense/oxidative stress and pulmonary DNA damage. Overall, lung injury was reduced using HFOV or CMV with iNO. (C) 2013 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 08/08199-2 - Effect of different mechanical ventilation techniques on the pulmonary oxidative stress induced by a model of acute respiratory distress syndrome in rabbits
• Grantee: Carlos Fernando Ronchi
• Support Opportunities: Scholarships in Brazil - Doctorate