| Full text | |
| Author(s): |
Total Authors: 3
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| Affiliation: | [1] Univ Fed Sao Paulo, Disciplina Infectol, Lab Especial Micol, BR-04039032 Sao Paulo - Brazil
Total Affiliations: 1
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| Document type: | Review article |
| Source: | Fungal Genetics and Biology; v. 61, p. 33-41, DEC 2013. |
| Web of Science Citations: | 14 |
| Abstract | |
Recently, Candida rugosa was characterized as a species complex comprising four taxa: C rugosa sensu stricto, Candida pseudorugosa, Candida neorugosa and Candida mesorugosa. Although considered relatively rare, several clusters of candidemia due to C rugosa complex had been reported presenting mortality rates close to 70%. In this work we discuss the systematization, phenotyping and molecular methods based on internal transcribed spacer region (ITS) sequencing and proteomic analyses for species identification, as well as clinical aspects of the C rugosa complex. We performed a Bayesian phylogenetic analysis using 72 ITS sequences representative of C rugosa complex isolates and related species within the genus. Biochemical, morphological and MALDI-TOF MS analyses were processed with C rugosa complex type strains and related species isolates. We described that the phylogeny showed four distinct clades inferred with high posterior probabilities, corresponding to the four species within the C. rugosa complex, excluding C. pararugosa. Biochemical and morphological aspects distinguished only C rugosa sensu strict but were not sufficient to accurately identify species within the rest of the complex. Protein spectrum profiles differentiated all reference strains from different species analyzed. To our knowledge, we presented the first phylogenetic analysis using a large collection of ITS sequences as well as proteomic profiles generated from isolates of the C rugosa complex and related species that can enlighten systematics, diagnostics and clinical research fields. (C) 2013 Elsevier Inc. All rights reserved. (AU) | |
| FAPESP's process: | 07/08575-1 - Hematogenic candidiasis: a multidisciplinary approach for the improvement of diagnostic tools and caracterization of biomarkers related to its prognosis |
| Grantee: | Arnaldo Lopes Colombo |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 09/10155-6 - Development and clinical validation of molecular methods for early diagnosis of invasive candidiasis and aspergillosis |
| Grantee: | Analy Salles de Azevedo Melo |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |