MicroRNA expression profile in head and neck cancer: HOX-cluster embedded microRNA-196a and microRNA-10b dysregulation implicated in cell proliferation

Author(s):Show less -

Severino, Patricia ^[1] ; Bruggemann, Holger ^[2] ; Andreghetto, Flavia Maziero ^[1] ; Camps, Carme ^[3] ; Garrido Klingbeil, Maria de Fatima ^[4] ; de Pereira, Welbert Oliveira ^[1] ; Soares, Renata Machado ^[1] ; Moyses, Raquel ^[5] ; Wuensch-Filho, Victor ^[6] ; Mathor, Monica Beatriz ^[4] ; Nunes, Fabio Daumas ^[7] ; Ragoussis, Jiannis ^[2] ; Tajara, Eloiza Helena ^[8]

Affiliation

^[1] Hosp Israelita Albert Einstein, Albert Einstein Res & Educ Inst, Sao Paulo - Brazil

^[2] Aarhus Univ, Dept Biomed, Aarhus - Denmark

^[3] Univ Oxford, Wellcome Trust Ctr Human Genet, Genom Grp, Oxford - England

^[4] CNEN, Nucl & Energet Res Inst IPEN, Radiat Technol Ctr CTR, Sao Paulo - Brazil

^[5] Univ Sao Paulo, Sch Med, Dept Surg, Div Head & Neck Surg, Sao Paulo - Brazil

^[6] Univ Sao Paulo, Fac Publ Hlth, Dept Epidemiol, Sao Paulo - Brazil

^[7] Univ Sao Paulo, Fac Dent, Dept Stomatol, Sao Paulo - Brazil

^[8] Sch Med, Dept Mol Biol, Sao Jose Do Rio Preto, SP - Brazil

• Document type: Journal article
• Source: BMC CANCER; v. 13, NOV 9 2013.
• Web of Science Citations: 37

Abstract

Background: Current evidence implicates aberrant microRNA expression patterns in human malignancies; measurement of microRNA expression may have diagnostic and prognostic applications. Roles for microRNAs in head and neck squamous cell carcinomas (HNSCC) are largely unknown. HNSCC, a smoking-related cancer, is one of the most common malignancies worldwide but reliable diagnostic and prognostic markers have not been discovered so far. Some studies have evaluated the potential use of microRNA as biomarkers with clinical application in HNSCC. Methods: MicroRNA expression profile of oral squamous cell carcinoma samples was determined by means of DNA microarrays. We also performed gain-of-function assays for two differentially expressed microRNA using two squamous cell carcinoma cell lines and normal oral keratinocytes. The effect of the over-expression of these molecules was evaluated by means of global gene expression profiling and cell proliferation assessment. Results: Altered microRNA expression was detected for a total of 72 microRNAs. Among these we found well studied molecules, such as the miR-17-92 cluster, comprising potent oncogenic microRNA, and miR-34, recently found to interact with p53. HOX-cluster embedded miR-196a/b and miR-10b were up-and down-regulated, respectively, in tumor samples. Since validated HOX gene targets for these microRNAs are not consistently deregulated in HNSCC, we performed gain-of-function experiments, in an attempt to outline their possible role. Our results suggest that both molecules interfere in cell proliferation through distinct processes, possibly targeting a small set of genes involved in cell cycle progression. Conclusions: Functional data on miRNAs in HNSCC is still scarce. Our data corroborate current literature and brings new insights into the role of microRNAs in HNSCC. We also show that miR-196a and miR-10b, not previously associated with HNSCC, may play an oncogenic role in this disease through the deregulation of cell proliferation. The study of microRNA alterations in HNSCC is an essential step to the mechanistic understanding of tumor formation and could lead to the discovery of clinically relevant biomarkers. (AU)

• FAPESP's process: 09/04166-5 - Characterization of microRNA regulatory networks involved in head and neck squamous cell carcinoma by means of microarray and functional studies in cell culture
• Grantee: Patricia Severino
• Support Opportunities: Regular Research Grants