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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Genetic and biochemical markers of hydroxyurea therapeutic response in sickle cell anemia

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Author(s):
Humberto Silva, Danilo Grunig [1, 2] ; Belini Junior, Edis [2] ; de Souza Carrocini, Gisele Cristine [2] ; Torres, Lidiane de Souza [2] ; Ricci Junior, Octavio [3] ; de Castro Lobo, Clarisse Lopes [4] ; Bonini-Domingos, Claudia Regina [2] ; de Almeida, Eduardo Alves [1]
Total Authors: 8
Affiliation:
[1] Sao Paulo State Univ, Dept Chem & Environm Sci, UNESP, Sao Paulo - Brazil
[2] Sao Paulo State Univ, Dept Biol, Hemoglobin & Hematol Genet Dis Lab, UNESP, Sao Paulo - Brazil
[3] Sao Jose do Rio Preto Med Sch FAMERP, Dept Med, Sao Paulo - Brazil
[4] Hematol State Inst Arthur de Siqueira Cavalcanti, HEMORIO, Rio De Janeiro - Brazil
Total Affiliations: 4
Document type: Journal article
Source: BMC MEDICAL GENETICS; v. 14, OCT 9 2013.
Web of Science Citations: 9
Abstract

Background: Sickle cell anemia (SCA) presents a complex pathophysiology which can be affected by a number of modifying factors, including genetic and biochemical ones. In Brazil, there have been no studies verifying beta(S)-haplotypes effect on oxidative stress parameters. This study evaluated beta(S)-haplotypes and Hb F levels effects on oxidative stress markers and their relationship with hydroxyurea (HU) treatment in SCA patients. Methods: The studied group was composed by 28 SCA patients. Thirteen of these patients were treated with HU and 15 of them were not. We used molecular methodology (PCR-RFLP) for hemoglobin S genotype confirmation and haplotypes identification. Biochemical parameters were measured using spectrophotometric methods (Thiobarbituric-acid-reactive substances and Trolox equivalent antioxidant capacity levels, catalase and GST activities) and plasma glutathione levels by High-performance liquid chromatography coupled to electrochemical detection. Results: We found the highest frequency of Bantu haplotype (48.2%) which was followed by Benin (32.1%). We observed also the presence of Cameroon haplotype, rare in Brazilian population and 19.7% of atypical haplotypes. The protective Hb F effect was confirmed in SCA patients because these patients showed an increase in Hb F levels that resulted in a 41.3% decrease on the lipid peroxidation levels (r=-0.74, p=0.01). Other biochemical parameters have not shown differential expression according to patient's haplotypes. Bantu haplotype presence was related to the highest lipid peroxidation levels in patients (p<0,01), but it also conferred a differential response to HU treatment, raising Hb F levels in 52.6% (p=0.03) when compared with the group with the same molecular profile without HU usage. Conclusions: SCA patients with Bantu haplotype showed the worst oxidative status. However these patients also demonstrated a better response to the treatment with HU. Such treatment seems to have presented a ``haplotype-dependent{''} pharmacological effect. (AU)