Analysis of Inteins in the Candida parapsilosis Co... - BV FAPESP
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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Analysis of Inteins in the Candida parapsilosis Complex for Simple and Accurate Species Identification

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Author(s):
Rocha Prandini, Tamara Heloisa [1] ; Theodoro, Raquel Cordeiro [2] ; Bruder-Nascimento, Ariane C. M. O. [1] ; Scheel, Christina M. [3] ; Bagagli, Eduardo [1]
Total Authors: 5
Affiliation:
[1] UNESP, Inst Biosci, Dept Microbiol & Immunol, Botucatu, SP - Brazil
[2] Univ Fed Rio Grande do Norte, Rio Grande Do Norte - Brazil
[3] Ctr Dis Control & Prevent, Atlanta, GA - USA
Total Affiliations: 3
Document type: Journal article
Source: Journal of Clinical Microbiology; v. 51, n. 9, p. 2830-2836, SEP 2013.
Web of Science Citations: 13
Abstract

Inteins are coding sequences that are transcribed and translated with flanking sequences and then are excised by an autocatalytic process. There are two types of inteins in fungi, mini-inteins and full-length inteins, both of which present a splicing domain containing well-conserved amino acid sequences. Full-length inteins also present a homing endonuclease domain that makes the intein a mobile genetic element. These parasitic genetic elements are located in highly conserved genes and may allow for the differentiation of closely related species of the Candida parapsilosis (psilosis) complex. The correct identification of the three psilosis complex species C. parapsilosis, Candida metapsilosis, and Candida orthopsilosis is very important in the clinical setting for improving antifungal therapy and patient care. In this work, we analyzed inteins that are present in the vacuolar ATPase gene VMA and in the threonyl-tRNA synthetase gene ThrRS in 85 strains of the Candida psilosis complex (46 C. parapsilosis, 17 C. metapsilosis, and 22 C. orthopsilosis). Here, we describe an accessible and accurate technique based on a single PCR that is able to differentiate the psilosis complex based on the VMA intein. Although the ThrRS intein does not distinguish the three species of the psilosis complex by PCR product size, it can differentiate them by sequencing and phylogenetic analysis. Furthermore, this intein is unusually present as both mini-and full-length forms in C. orthopsilosis. Additional population studies should be performed to address whether this represents a common intraspecific variability or the presence of subspecies within C. orthopsilosis. (AU)

FAPESP's process: 12/07741-3 - ThrRS intein of species from the genus Candida: phylogeny, potential molecular marker and therapeutic target
Grantee:Eduardo Bagagli
Support Opportunities: Regular Research Grants
FAPESP's process: 12/04003-1 - ThrRS intein of species from the genus Candida: phylogeny, potential molecular marker and therapeutic target
Grantee:Tâmara Heloísa Rocha Prandini
Support Opportunities: Scholarships in Brazil - Doctorate