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Polymorphism identification and genotyping of Candida orthopsilosis using restriction site associated DNA (RAD) markers

Grant number: 14/04811-6
Support Opportunities:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): July 01, 2014
Effective date (End): December 31, 2014
Field of knowledge:Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
Principal Investigator:Eduardo Bagagli
Grantee:Tâmara Heloísa Rocha Prandini
Supervisor: Geraldine Butler
Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
Research place: University College Dublin, Ireland  
Associated to the scholarship:12/04003-1 - ThrRS intein of species from the genus Candida: phylogeny, potential molecular marker and therapeutic target, BP.DR

Abstract

The incidence of fungal infections due to C. parapsilosis and closely related species (Candida psilosis complex) has increased in the last few years and due to the genomic researches it has been possible to note the differences among these species complex, which may have an impact on the treatment of candidemias and comprehension of the evolutionary history of these species. Several studies have identified heterogeneity among C. orthopsilosis isolates. Recently we have found the existence of two idiomorphic inteins, ThrRS-A (mini-intein) and ThrRS-B (full length), in the same insertion site of distinct individuals of C. orthopsilosis species, which, alternatively, may indicate the occurrence of two distinct species among C. orthopsilosis isolates. To evaluate the biological meaning of such observation, as well as the meaning of other polymorphic markers, such as MTL (Mating Type Loci) and ITS region profiles, already described for this species, we propose to genotype this isolates by Restriction Site Associated DNA (RAD tag) which are short fragments of DNA adjacent to each instance of a particular restriction enzyme recognition site, which is a novel and efficient technique based on Next Generation Sequencing. The phylogenetic knowledge of C. orthopsilosis species by using such powerful molecular technique may have impact in the correct treatment of candidemias and epidemiological studies. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SCHRODER, MARKUS S.; DE SAN VICENTE, KONTXI MARTINEZ; PRANDINI, TAMARA H. R.; HAMMEL, STEPHEN; HIGGINS, DESMOND G.; BAGAGLI, EDUARDO; WOLFE, KENNETH H.; BUTLER, GERALDINE. Multiple Origins of the Pathogenic Yeast Candida orthopsilosis by Separate Hybridizations between Two Parental Species. PLoS Genetics, v. 12, n. 11, . (14/04811-6)
SCHRODER, MARKUS S.; DE SAN VICENTE, KONTXI MARTINEZ; PRANDINI, TAMARA H. R.; HAMMEL, STEPHEN; HIGGINS, DESMOND G.; BAGAGLI, EDUARDO; WOLFE, KENNETH H.; BUTLER, GERALDINE. Multiple Origins of the Pathogenic Yeast Candida orthopsilosis by Separate Hybridizations between Two Parental Species. PLOS GENETICS, v. 12, n. 11, p. 25-pg., . (14/04811-6)

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